Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii

被引：446

作者：

Su, C. ^{[1
]}

Shwab, E. K. ^{[1
]}

Zhou, P. ^{[2
]}

Zhu, X. Q. ^{[2
]}

Dubey, J. P. ^{[3
]}

机构：

[1] Univ Tennessee, Dept Microbiol, Knoxville, TN 37966 USA

[2] S China Agr Univ, Parasitol Lab, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

[3] USDA, Anim Parasit Dis Lab, Beltsville, MD 20705 USA

来源：

PARASITOLOGY | 2010年 / 137卷 / 01期

关键词：

Toxoplasma gondii; genetic characterization; molecular detection and identification; REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; TRANSFER HYBRIDIZATION PROBES; RACCOONS PROCYON-LOTOR; FREE-RANGE CHICKENS; GENETIC-CHARACTERIZATION; HIGH-RESOLUTION; AMNIOTIC-FLUID; IMMUNOCOMPROMISED PATIENTS; CONGENITAL TOXOPLASMOSIS;

D O I：

10.1017/S0031182009991065

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The development of simple, sensitive and rapid methods for the detection and identification of Toxoplasma gondii is important for the diagnosis and epidemiological studies of the zoonotic disease toxoplasmosis. In the past 2 decades, molecular methods based on a variety of genetic markers have been developed, each with its advantages and limitations. The application of these methods has generated invaluable information to enhance Our understanding of the epidemiology, population genetics and phylogeny of T. gondii. However, since most studies focused solely on the detection but not genetic characterization of T. gondii, the information obtained was limited. In this review, we discuss some widely used molecular methods and propose an integrated approach for the detection and identification of T. gondii, in order to generate maximum information for epidemiological, population and phylogenetic studies of this key pathogen.

引用

页码：1 / 11

页数：11

共 69 条

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