Confocal laser scanning microscopy

被引：127

作者：

Paddock, SW ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Mol Biol, Madison, WI 53706 USA

来源：

BIOTECHNIQUES | 1999年 / 27卷 / 05期

关键词：

D O I：

10.2144/99275ov01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Many technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued development in digital imaging methods, laser technology and the availability of brighter and more photostable fluorescent probes. Such advances have made possible novel experimental approaches for multiple label fluorescence, live cell imaging and multidimensional microscopy.

引用

页码：992 / +

页数：10

共 69 条

[51] Vital imaging: Two photons are better than one [J].