Occurrence and determination of a natriuretic hormone, 2,7,8-trimethyl-2-(β-carboxyethyl)-6-hydroxy chroman, in rat plasma, urine, and bile

被引：36

作者：

Hattori, A ^{[1
]}

Fukushima, T ^{[1
]}

Imai, K ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Bioanalyt Chem, Bunkyo Ku, Tokyo 1130033, Japan

来源：

ANALYTICAL BIOCHEMISTRY | 2000年 / 281卷 / 02期

关键词：

LLU-alpha; gamma-CEHC; DBD-PZ; fluorometric detection; coupled-column HPLC; chiral column;

D O I：

10.1006/abio.2000.4566

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The occurrence of a new natriuretic hormone, 2,7,8-trimethyl-2-(beta-carboxyethyl)-6-hydroxy chroman (LLU-alpha; gamma-CEHC) in rat plasma was demonstrated and its concentration was determined using a coupled-column HPLC with a fluorometric derivatization with 4-N,N-dimethylaminosulfonyl-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) followed by O-acetylation, The concentration of LLU-alpha was 328 +/- 113 nM in rat plasma (N = 5). LLU-alpha was found in not only urine, but also bile, suggesting an enterohepatic circulation in body, We also assigned the configuration at C-2 of LLU-alpha in these biological fluids as (S)-form by an HPLC with a chiral column. The LLU-alpha concentration decreased significantly by fasting for 3 days (P < 0.01). These results support the possibility that LLU-alpha is produced from gamma-tocopherol in diet via oxidative metabolism without racemization, (C) 2000 Academic Press.

引用

页码：209 / 215

页数：7

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