Multiple overlapping epitopes in the repetitive unit of the shed acute-phase antigen from Trypanosoma cruzi enhance its immunogenic properties

被引：29

作者：

Alvarez, P

Leguizamón, MS

Buscaglia, CA

Pitcovsky, TA

Campetella, O

机构：

[1] Univ Nacl Gen San Martin, Inst Invest Biotecnol, Predio INTI, RA-1650 San Martin, Buenos Aires, Argentina

[2] Univ Buenos Aires, Fac Med, Dept Microbiol, Buenos Aires, DF, Argentina

来源：

INFECTION AND IMMUNITY | 2001年 / 69卷 / 12期

关键词：

D O I：

10.1128/IAI.69.12.7946-7949.2001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The repetitive shed acute-phase antigen (SAPA) from Trypanosoma cruzi was thoroughly mapped by SPOT peptides and phage display strategies, showing that a single SAPA repeat is composed of multiple overlapping B-cell epitopes. We propose that this intricate antigenic structure constitutes an alternative device to repetitiveness in order to improve its immunogenicity.

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页码：7946 / 7949

页数：4

相关论文

共 25 条

[1] IDENTIFICATION OF A TRYPANOSOMA-CRUZI ANTIGEN THAT IS SHED DURING THE ACUTE PHASE OF CHAGAS-DISEASE [J].

AFFRANCHINO, JL ;

IBANEZ, CF ;

LUQUETTI, AO ;

RASSI, A ;

REYES, MB ;

MACINA, RA ;

ASLUND, L ;

PETTERSSON, U ;

FRASCH, ACC .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1989, 34 (03) :221-228

[2] The repetitive domain of Trypanosoma cruzi trans-sialidase enhances the immune response against the catalytic domain [J].

Buscaglia, CA ;

Campetella, O ;

Leguizamón, MS ;

Frasch, ACC .

JOURNAL OF INFECTIOUS DISEASES, 1998, 177 (02) :431-436

[3] TRYPANOSOMA-CRUZI TRANS-SIALIDASE - ENHANCEMENT OF VIRULENCE IN A MURINE MODEL OF CHAGAS-DISEASE [J].

CHUENKOVA, M ;

PEREIRA, MEA .

JOURNAL OF EXPERIMENTAL MEDICINE, 1995, 181 (05) :1693-1703

[4] SIMULTANEOUS MULTIPLE PEPTIDE-SYNTHESIS UNDER CONTINUOUS-FLOW CONDITIONS ON CELLULOSE PAPER DISKS AS SEGMENTAL SOLID SUPPORTS [J].

FRANK, R ;

DORING, R .

TETRAHEDRON, 1988, 44 (19) :6031-6040

[5]

Gao W, 2001, EUR J IMMUNOL, V31, P1503, DOI 10.1002/1521-4141(200105)31:5&lt

[6]

1503::AID-IMMU1503&gt

[7]

3.0.CO

[8]

2-W

[9] Dominant T- and B-cell epitopes in an autoantigen linked to Chagas' disease [J].

Gironès, N ;

Rodríguez, CI ;

Carrasco-Marín, E ;

Hernáez, RF ;

de Rego, JL ;

Fresno, M .

JOURNAL OF CLINICAL INVESTIGATION, 2001, 107 (08) :985-993

[10] Variation in repeat number within the alpha C protein of group B streptococci alters antigenicity and protective epitopes [J].

Gravekamp, C ;

Horensky, DS ;

Michel, JL ;

Madoff, LC .

INFECTION AND IMMUNITY, 1996, 64 (09) :3576-3583