Quantitative analysis of the regulation of leukocyte chemosensory migration by a vascular prosthetic biomaterial

被引:7
作者
Chang, CC [1 ]
Lieberman, SM [1 ]
Moghe, PV [1 ]
机构
[1] Rutgers State Univ, Dept Chem & Biochem Engn, Piscataway, NJ 08854 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1023/A:1008925722623
中图分类号
R318 [生物医学工程];
学科分类号
0831 [生物医学工程];
摘要
The need for improved, infection-resistant vascular biomaterials calls for more objective evaluation of the immune pathophysiology of implantable prosthetic materials. In this study we have developed a new strategy to quantitatively characterize population-averaged responses of immune cell migration on vascular prosthetic materials. This approach, incorporating a chemokinetically regulated "biomaterial-gel" sandwich configuration, was applied to quantify both random and directed modes of the chemosensory migration of human neutrophil leukocytes on expanded polytetrafluoroethylene (ePTFE). Our studies show that (a) microporous, synthetic materials like ePTFE suppress the basal rate of random cell migration relative to that reported on non-porous control surfaces; (b) stimulation with chemoattractant (formyl peptide) can significantly elevate rates of random and directed migration on ePTFE; and (c) protein conditioning of ePTFE with albumin or immunoglobulin G can differentially modulate the rates and relative proportion of random and directional components of leukocyte migration response to chemoattractant. This, to our knowledge, is the first objective quantitation of chemokinetically regulated cell migration on implantable prosthetic materials. (C) 2000 Kluwer Academic Publishers.
引用
收藏
页码:337 / 344
页数:8
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