Cell penetrating silica nanoparticles doped with two-photon absorbing fluorophores

被引:26
作者
Bertazza, Loris
Celotti, Lucia
Fabbrini, Graziano
Loi, Maria Antonietta
Maggini, Michele
Mancin, Fabrizio
Marcuz, Silvia
Menna, Enzo
Muccini, Michele
Tonellato, Umberto
机构
[1] Univ Padua, Dipartimento Sci Chim, I-35131 Padua, Italy
[2] Univ Padua, Dipartimento Biol, I-35131 Padua, Italy
[3] CNR, Ist Studio Mat Nanostrutturati, I-40129 Bologna, Italy
[4] Univ Groningen, Ctr Mat Sci, NL-9747 AG Groningen, Netherlands
关键词
silica nanoparticles; two-photon absorption; fluorescent dyes; cellular uptake;
D O I
10.1016/j.tet.2006.08.044
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
Organosilica nanoparticles, doped with two-photon absorbing distyrylbenzene derivatives, were prepared and studied as cell staining agents. Two dyes were used, bearing either two peripheral dimethylamino groups or one dimethylamino and one cyano group. Due to the internal charge transfer character of their excited state, the dyes employed show a red-shifted quenched emission in polar solvents. Once included in the particles, the properties of the two dyes undergo a substantial variation. Particles doped with the cyano substituted distyrylbenzene show a remarkable emission quantum yield in water, probably due to solvent exclusion from the nanoparticle core. To the contrary, the emission of the particles containing the dye substituted with two dimethylamino groups is substantially quenched. Fluorescence emission induced by two-photon absorption follows the same behaviour. The doped nanoparticles can be rapidly internalized by tumour cells with accumulation limited to the cytoplasm and show no cytotoxicity at low concentrations. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:10434 / 10440
页数:7
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