The adrenal genetic puzzle: How do the fetal and adult pieces differ?

被引:21
作者
Rainey, WE [1 ]
Parker, CR
Rehman, K
Carr, BR
机构
[1] Univ Texas, SW Med Ctr, Dept Obstet & Gynecol, Div Reprod Endocrinol, Dallas, TX 75390 USA
[2] Univ Alabama Birmingham, Dept Obstet & Gynecol, Birmingham, AL 35294 USA
关键词
D O I
10.1081/ERC-120016974
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The basis for the steroidogenic differences between the human fetal adrenal (HFA) and adult adrenal is not well defined. However, gene expression clearly plays a critical role in defining their distinct steroidogenic and structural phenotypes. We used DNA microarrays to compare expression levels of several thousand transcripts between the HFA and adult adrenal gland. Gene profiling was done using seven independent microarrays that contained between 7075 and 9182 cDNA elements. Twenty-five transcripts were found to have a greater than 5-fold difference in expression between HFA and adult adrenals. The largest differences were observed for transcripts that encode insulin-like growth factor-II (IGF-II) (25-fold higher in HFA) and 3beta hydroxysteroid dehydrogenase (3betaHSD) (21-fold higher in adult). The vast majority of the 25 transcripts have not been studied with regard to adrenal function. We also determined the transcripts that had the highest signal intensities, which is an approximate measure of expression level, for both the fetal and adult adrenal RNA samples. The enzyme 24-dehydrocholesterol reductase, which is involved in cholesterol biosynthesis, exhibited the highest signal intensity for fetal adrenal RNA. For adult adrenal mRNA, the expression of 11beta-hydroxylase transcripts was found to have the highest signal intensity ranking. Overall, 10 of the top 20 highest signal intensities were similar for adult and fetal adrenal transcripts. The gene profile data for fetal vs. adult adrenal glands should provide valuable information that could help define mechanisms involved in adrenal growth and development.
引用
收藏
页码:611 / 622
页数:12
相关论文
共 18 条
[1]  
BASSETT MH, 2002, 84 END SOC C
[2]   CHOLESTEROL-SYNTHESIS IN HUMAN-FETAL TISSUES [J].
CARR, BR ;
SIMPSON, ER .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1982, 55 (03) :447-452
[3]   ENDOCRINE AND NEUROGENIC REGULATION OF THE ORPHAN NUCLEAR RECEPTORS NUR77 AND NURR-1 IN THE ADRENAL-GLANDS [J].
DAVIS, IJ ;
LAU, LF .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (05) :3469-3483
[4]   3-BETA-HYDROXYSTEROID DEHYDROGENASE ISOMERASE IN THE FETAL ZONE AND NEOCORTEX OF THE HUMAN FETAL ADRENAL-GLAND [J].
DOODY, KM ;
CARR, BR ;
RAINEY, WE ;
BYRD, W ;
MURRY, BA ;
STRICKLER, RC ;
THOMAS, JL ;
MASON, JI .
ENDOCRINOLOGY, 1990, 126 (05) :2487-2492
[5]  
EBERT SN, 1994, J BIOL CHEM, V269, P20885
[6]   EXPRESSION OF SOMATOMEDIN INSULIN-LIKE GROWTH-FACTOR MESSENGER RIBONUCLEIC-ACIDS IN THE HUMAN-FETUS - IDENTIFICATION, CHARACTERIZATION, AND TISSUE DISTRIBUTION [J].
HAN, VKM ;
LUND, PK ;
LEE, DC ;
DERCOLE, AJ .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1988, 66 (02) :422-429
[7]   Insulin-like growth factors enhance steroidogenic enzyme and corticotropin receptor messenger ribonucleic acid levels and corticotropin steroidogenic responsiveness in cultured human adrenocortical cells [J].
LAllemand, D ;
Penhoat, A ;
Lebrethon, MC ;
Ardevol, R ;
Baehr, V ;
Oelkers, W ;
Saez, JM .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1996, 81 (11) :3892-3897
[8]   Promoter function of the angiogenic inducer Cyr61 gene in transgenic mice:: Tissue specificity, inducibility during wound healing, and role of the serum response element [J].
Latinkic, BV ;
Mo, FE ;
Greenspan, JA ;
Copeland, NG ;
Gilbert, DJ ;
Jenkins, NA ;
Ross, SR ;
Lau, LF .
ENDOCRINOLOGY, 2001, 142 (06) :2549-2557
[9]   MITOGENIC ACTION, REGULATION, AND LOCALIZATION OF INSULIN-LIKE GROWTH-FACTORS IN THE HUMAN FETAL ADRENAL-GLAND [J].
MESIANO, S ;
MELLON, SH ;
JAFFE, RB .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1993, 76 (04) :968-976
[10]   Developmental and functional biology of the primate fetal adrenal cortex [J].
Mesiano, S ;
Jaffe, RB .
ENDOCRINE REVIEWS, 1997, 18 (03) :378-403