Powdery Mildew-Infection Changes Bacterial Community Composition in the Phyllosphere

被引:39
作者
Suda, Wataru [1 ]
Nagasaki, Asami [1 ]
Shishido, Masahiro [1 ]
机构
[1] Chiba Univ, Grad Sch Hort, Matsudo, Chiba 2718510, Japan
基金
日本学术振兴会;
关键词
powdery mildew; phyllosphere; epiphytic bacteria; denaturing gradient gel electrophoresis (DGGE); community-level physiological profile (CLPP); ARBUSCULAR MYCORRHIZAL FUNGUS; MICROBIAL COMMUNITIES; PSEUDOMONAS-SYRINGAE; SEASONAL-CHANGES; PLANT; POPULATIONS; MICROORGANISMS; COMPLEX; DNA;
D O I
10.1264/jsme2.ME09114
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To investigate changes in bacterial communities associated with a fungal foliar disease, epiphytic bacteria from powdery mildew-infected and uninfected leaves of cucumber and Japanese spindle were analyzed using both culture-dependent and- independent methods. Dilution plate counting suggested that powdery mildew-infected leaves likely accommodated larger populations of phyllosphere bacteria than uninfected leaves. Community-level physiological profiles (CLPP) also indicated that functional diversity, richness, and evenness of bacterial communities were significantly greater in the phyllosphere of powdery mildew-infected leaves. Genotype diversity and richness based on band patterns of denaturing gradient gel electrophoresis (DGGE) of the phyllosphere bacterial community were greater for leaves infected by powdery mildew. A principle component analysis of CLPP and DGGE patterns revealed a clear difference between infected and uninfected leaves of both plant species. These results suggest that powdery mildew-infection results in larger bacterial populations, and greater diversity and richness, and also changes the structure of the phyllosphere bacterial community. Furthermore, DNA sequences of the DGGE bands that showed greater intensity in the infected than uninfected leaves, differed between cucumber and Japanese spindle. This suggests that specific bacteria are associated with the plant species accompanying this fungal infection.
引用
收藏
页码:217 / 223
页数:7
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