Assessment and reduction of comet assay variation in relation to DNA damage: studies from the European Comet Assay Validation Group

被引：81

作者：

Moller, Peter ^{[1
]}

Moller, Lennart ^{[2
]}

Godschalk, Roger W. L. ^{[3
]}

Jones, George D. D. ^{[4
]}

机构：

[1] Univ Copenhagen, Dept Publ Hlth, Environm Hlth Sect, Oster Farimagsgade 5,Bldg 5,2nd Floor,POB 2099, DK-1014 Copenhagen K, Denmark

[2] Karolinska Inst, Dept Biosci & Nutr, Huddinge, Sweden

[3] Maastricht Univ, Dept Hlth Risk Anal & Toxicol, Maastricht, Netherlands

[4] Univ Leicester, Dept Canc Studies & Mol Med, Leicester, Leics, England

来源：

MUTAGENESIS | 2010年 / 25卷 / 02期

关键词：

SCORING EXERCISE; BASE OXIDATION;

D O I：

10.1093/mutage/gep067

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The alkaline single cell gel electrophoresis (comet) assay has become a widely used method for the detection of DNA damage and repair in cells and tissues. Still, it has been difficult to compare results from different investigators because of differences in assay conditions and because the data are reported in different units. The European Comet Assay Validation Group (ECVAG) was established for the purpose of validation of the comet assay with respect to measures of DNA damage formation and its repair. The results from this inter-laboratory validation trail showed a large variation in measured level of DNA damage and formamidopyrimidine DNA glycosylase-sensitive sites but the laboratories could detect concentration-dependent relationships in coded samples. Standardization of the results with reference standards decreased the inter-laboratory variation. The ECVAG trail indicates substantial reliability for the measurement of DNA damage by the comet assay but there is still a need for further validation to reduce both assay and inter-laboratory variation.

引用

页码：109 / 111

页数：3

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