Polymorphic microsatellite markers for studies of Aedes aegypti (Diptera: Culicidae), the vector of dengue and yellow fever

被引：61

作者：

Slotman, M. A. ^{[1
]}

Kelly, N. . B.

Harrington, L. . C.

Kitthawee, S.

Jones, J. . W.

Scott, T. . W.

Caccone, A.

Powell, J. . R.

机构：

[1] Yale Univ, Dept Ecol & Evolutionary Biol, New Haven, CT 06250 USA

[2] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA

[3] Mahidol Univ, Fac Sci, Dept Biol, Bangkok 10400, Thailand

[4] Armed Forces Inst Med Sci, US Med Component, Dept Entomol, Bangkok 10400, Thailand

[5] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA

来源：

MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 01期

关键词：

Aedes aegypti; dengue vector; genetic markers; microsatellites; population genetics; POPULATION-GENETICS; LOCI;

D O I：

10.1111/j.1471-8286.2006.01533.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A significant challenge to population genetic studies of the dengue vector, Aedes aegypti, has been the lack of polymorphic microsatellite loci. In an effort to develop useful markers, we evaluated the genetic variation at 17 microsatellite loci identified in the A. aegypti genome. Nine loci with at least five alleles were identified in field-collected specimens from Thailand. An additional two loci carried five alleles if samples from an A. aegypti laboratory colony were included. Our results greatly increase the number of highly variable markers available for the study of the genetics and the population structure of this medically important species.

引用

页码：168 / 171

页数：4

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