Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on hepatitis A virus

被引：10

作者：

Kiyohara, Tomoko ^{[1
]}

Totsuka, Atsuko ^{[1
]}

Yoneyama, Tetsuo ^{[1
]}

Ishii, Koji ^{[1
]}

Ito, Toshihiro ^{[2
]}

Wakita, Takaji ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Virol 2, Tokyo 2080011, Japan

[2] Tottori Univ, Fac Agr, Lab Vet Publ Hlth, Tottori 6808553, Japan

来源：

ARCHIVES OF VIROLOGY | 2009年 / 154卷 / 08期

关键词：

IDIOTYPE VACCINES; NEUTRALIZATION; MUTANTS;

D O I：

10.1007/s00705-009-0433-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two anti-idiotypic monoclonal antibodies (mAb2s; named 94-2 and 94-7), were generated from a BALB/c mouse immunized with human monoclonal anti-hepatitis A virus (HAV) neutralizing antibody KF94. We characterized the properties of the mAb2s and determined interactions between mAb2s, KF94 and HAV using enzyme-linked immunosorbent assay, immunofluorescence assay and HAV infectivity assay. Inactivated HAV inhibited mAb2 binding to KF94, indicating that the mAb2s mimicked the HAV neutralization site that was complementary to the paratope of KF94. MAb2 94-7 competed with an anti-HAV cellular receptor antibody for binding to HAV-susceptible cells and partially blocked virus infection. We speculated that mAb2 94-7 mimicked a portion of the HAV receptor-binding site. The ability to generate mAb2 implies that HAV receptor-binding sites are exposed on the surface of HAV, permitting antibody access.

引用

页码：1263 / 1269

页数：7

共 22 条

[1] Idiotype vaccines: Forgotten but not gone [J].

Bona, CA .

NATURE MEDICINE, 1998, 4 (06) :668-669

[2]

BONA CA, 1984, MONOCLONAL ANTIIDIOT, P141

[3] Antibody-mediated neutralization of human rhinovirus 14 explored by means of cryoelectron microscopy and X-ray crystallography of virus-fab complexes [J].

Che, ZW ;

Olson, NH ;

Leippe, D ;

Lee, WM ;

Mosser, AG ;

Rueckert, RR ;

Baker, TS ;

Smith, TJ .

JOURNAL OF VIROLOGY, 1998, 72 (06) :4610-4622

[4]

Chiba J, 1988, Hum Cell, V1, P31

[5] IODINATION OF HEPATITIS-A VIRUS REVEALS A 4TH STRUCTURAL POLYPEPTIDE [J].

COULEPIS, AG ;

LOCARNINI, SA ;

GUST, ID .

JOURNAL OF VIROLOGY, 1980, 35 (02) :572-574

[6] Analysis of the full-length genome of hepatitis A virus isolated in South America:: heterogeneity and evolutionary constraints [J].

Garcia-Aguirre, Laura ;

Cristina, Juan .

ARCHIVES OF VIROLOGY, 2008, 153 (08) :1473-1478

[7] IMMUNOREACTIVITY OF HUMAN AND RABBIT ANTISERA TO HEPATITIS-A VIRUS [J].

GAUSSMULLER, V ;

DEINHARDT, F .

JOURNAL OF MEDICAL VIROLOGY, 1988, 24 (02) :219-228

[8] Canyon rim residues, including antigenic determinants, modulate serotype-specific binding of polioviruses to mutants of the poliovirus receptor [J].

Harber, J ;

Bernhardt, G ;

Lu, HH ;

Sgro, JY ;

Wimmer, E .

VIROLOGY, 1995, 214 (02) :559-570

[9] IDIOTYPIC VACCINES AND INFECTIOUS-DISEASES [J].

HIERNAUX, JR .

INFECTION AND IMMUNITY, 1988, 56 (06) :1407-1413

[10]

HUGHES JV, 1979, J VIROL, V55, P395

← 1 2 3 →