Characterization of a petunia acetyltransferase involved in the biosynthesis of the floral volatile isoeugenol

被引：122

作者：

Dexter, Richard

Qualley, Anthony

Kish, Christine M.

Ma, Choong Je

Koeduka, Takao

Nagegowda, Dinesh A.

Dudareva, Natalia

Pichersky, Eran

Clark, David ^{[1
]}

机构：

[1] Univ Florida, Dept Environm Hort, Gainesville, FL 32611 USA

[2] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA

[3] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA

来源：

PLANT JOURNAL | 2007年 / 49卷 / 02期

关键词：

isoeugenol; floral volatiles; BAHD acyltransferase; coniferyl acetate; Petunia x hybrida;

D O I：

10.1111/j.1365-313X.2006.02954.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Petunia flower petals emit large amounts of isoeugenol, which has been shown to be synthesized by isoeugenol synthase (PhIGS1) from an ester of coniferyl alcohol, hypothesized to be coniferyl acetate. This paper describes the identification and characterization of a novel petunia gene encoding an enzyme belonging to the BAHD acyltransferase family whose expression correlates with isoeugenol biosynthesis. RNAi suppression of this gene results in inhibition of isoeugenol biosynthesis. Biochemical characterization of the protein encoded by this gene showed that it has acetyltransferase activity and is most efficient with coniferyl alcohol among the alcohol substrates tested. Overall, these data support the conclusion that coniferyl acetate is the substrate of isoeugenol synthase.

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收藏

页码：265 / 275

页数：11

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