Rapid switching of TFIIH between RNA polymerase I and II transcription and DNA repair in vivo

被引：172

作者：

Hoogstraten, D

Nigg, AL

Heath, H

Mullenders, LHF

van Driel, R

Hoeijmakers, JHJ

Vermeulen, W ^{[1
]}

Houtsmuller, AB

机构：

[1] Erasmus Univ, Dept Cell Biol & Genet, Ctr Med Genet, CBG, NL-3000 DR Rotterdam, Netherlands

[2] Erasmus Univ, Dept Pathol, Josephine Nefkens Inst, NL-3000 DR Rotterdam, Netherlands

[3] Leiden Univ, CBG, Ctr Med Genet, Dept Radiat Genet & Chem Mutagenesis, Leiden, Netherlands

[4] Univ Amsterdam, Swammerdam Inst Life Sci, Amsterdam, Netherlands

来源：

MOLECULAR CELL | 2002年 / 10卷 / 05期

关键词：

D O I：

10.1016/S1097-2765(02)00709-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transcription/repair factor TFIIH operates as a DNA helix opener in RNA polymerase II (RNAP2) transcription and nucleotide excision repair. To study TFIIH in vivo, we generated cell lines expressing functional GFP-tagged TFIIH. TFIIH was homogeneously distributed throughout the nucleus with nucleolar accumulations. We provide in vivo evidence for involvement of TFIIH in RNA polymerase I (RNAP1) transcription. Photobleaching revealed that TFIIH moves freely and gets engaged in RNAP1 and RNAP2 transcription for similar to25 and similar to6 s, respectively. TFIIH readily switches between transcription and repair sites (where it is immobilized for similar to4 min) without large-scale alterations in composition. Our findings support a model of diffusion and random collision of individual components that permits a quick and versatile response to changing conditions.

引用

页码：1163 / 1174

页数：12

共 41 条

[1] MAMMALIAN DNA NUCLEOTIDE EXCISION-REPAIR RECONSTITUTED WITH PURIFIED PROTEIN-COMPONENTS [J].