Characterization of 22 microsatellites loci from the endangered Houbara bustard (Chlamydotis undulata undulata)

被引:18
作者
Chbel, F [1 ]
Broderick, D
Idaghdour, Y
Korrida, A
McCormick, P
机构
[1] Int Fdn Conservat & Dev Wildlife, Genet Lab, Inezgane, Morocco
[2] Int Fdn Conservat & Dev Wildlife, Inseminat Lab, Inezgane, Morocco
来源
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 04期
关键词
Chlamydotis undulata undulata; desert-adapted birds; Houbara bustards; microsatellites; PCR; probability of exclusion;
D O I
10.1046/j.1471-8286.2002.00287.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Here we present a new set of 22 microsatellite loci isolated from Chlamydotis undulata undulata, an endangered Houbara bustard found across North Africa. The number of alleles per locus ranged from one to nine, and heterozygosities ranged from 0.167 to 0.944. Total exclusionary probabilities using these loci for the first and the second parent were 0.992932 and 0.999915, respectively. Successful cross-amplification was observed in eight other Otididae species (12-22 of the 22 loci). These microsatellite markers are powerful tools for genetic identification, paternity assignment and population genetic studies.
引用
收藏
页码:484 / 487
页数:4
相关论文
共 8 条
[1]  
BOTSTEIN D, 1980, AM J HUM GENET, V32, P314
[2]  
CRAMP S, 1993, BIRDS W PALEARCTIC, V4, P659
[3]   Taxonomy of the Houbara bustard Chlamydotis undulata subspecies considered on the basis of sexual display and genetic divergence [J].
Gaucher, P ;
Paillat, P ;
Chappuis, C ;
SaintJalme, M ;
Lotfikhah, F ;
Wink, M .
IBIS, 1996, 138 (02) :273-282
[4]  
JACQUIN, 1784, BEYTR GESCH VOGEL
[5]   Isolation and characterization of microsatellite loci in the great bustard, Otis tarda [J].
Lieckfeldt, D ;
Schmidt, A ;
Pitra, C .
MOLECULAR ECOLOGY NOTES, 2001, 1 (03) :133-134
[6]   Statistical confidence for likelihood-based paternity inference in natural populations [J].
Marshall, TC ;
Slate, J ;
Kruuk, LEB ;
Pemberton, JM .
MOLECULAR ECOLOGY, 1998, 7 (05) :639-655
[7]  
PEACOCK MM, IN PRESS MOL ECOLOGY
[8]  
Sambrook J., 1989, MOL CLONING