Optimization of solid-state medium for the production of inulinase by Kluyveromyces S120 using response surface methodology

被引：65

作者：

Xiong, Chen ^{[1
]}

Wang Jinhua ^{[1
]}

Li Dongsheng ^{[1
]}

机构：

[1] Hubei Univ Technol, Coll Bioengn, Hubei Key Lab Ind Microbiol, Wuhan 430068, Peoples R China

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2007年 / 34卷 / 02期

关键词：

response surface methodology; optimization; Kluyveromyces S120; inulinase production; solid-state fermentation;

D O I：

10.1016/j.bej.2006.12.012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The optimization of nutrient levels for the production of inulinase by a newly strain Kluyveromyces S120 in solid-state fermentation (SSF) was carried out using response surface methodology (RSM) based on Plackett-Burman design and Box-Behnken design. In the first optimization step, a Plackett-Burman design was used to evaluate the influence of related factors. Inulin, corn steep liquor and (NH4)(2)SO4 were found to be more compatible supplement with the substrate of wheat bran and positively influenced on inulinase production. In the second step, the concentrations of the three nutrients above were further optimized using a Box-Behnken design. The final concentration of medium optimized with RSM was 12.72% inulin, 10.76% corn steep liquor and 1.61% (NH4)(2)SO4 by employing wheat bran as the solid substrate. The average inulinase activity (409.8 U/g initial dry substrate) in triplicate under optimal medium was obtained. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：179 / 184

页数：6

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