Observation of single-stranded DNA on mica and highly oriented pyrolytic graphite by atomic force microscopy

被引：103

作者：

Adamcik, Jozef

Klinov, Dmitry V.

Witz, Guillaume

Sekatskii, Sergey K.

Dietler, Giovanni ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Phys Mat Vivante, CH-1015 Lausanne, Switzerland

[2] Safarik Univ, Fac Sci, Inst Chem, Dept Biochem, Kosice 04154, Slovakia

[3] Russian Acad Sci, MM Shemyakin Bioorgan Chem Inst, Moscow 117871, Russia

来源：

FEBS LETTERS | 2006年 / 580卷 / 24期

关键词：

atomic force microscopy; single-stranded DNA; double-stranded DNA; mica; highly oriented pyrolytic graphite;

D O I：

10.1016/j.febslet.2006.09.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Atomic force microscopy was used to image single-stranded DNA (ssDNA) adsorbed on mica modified by Mg2+, by 3-aminopropyltriethoxysilane or on modified highly oriented pyrolytic graphite (HOPG). ssDNA molecules on mica have compact structures with lumps, loops and super twisting, while on modified HOPG graphite ssDNA molecules adopt a conformation without secondary structures. We have shown that the immobilization of ssDNA under standard conditions on modified HOPG eliminates intramolecular base-pairing, thus this method could be important for studying certain processes involving ssDNA in more details. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5671 / 5675

页数：5

共 21 条

[1]

Adamcík J, 2002, ELECTROPHORESIS, V23, P3300, DOI 10.1002/1522-2683(200210)23:19<3300::AID-ELPS3300>3.0.CO

[2]

2-Q

[3]

Atomic force microscopy of DNA immobilized onto a highly oriented pyrolytic graphite electrode surface [J].