Limonene bioconversion to high concentrations of a single and stable product, perillic acid, by a solvent-resistant Pseudomonas putida strain

被引：52

作者：

Speelmans, G ^{[1
]}

Bijlsma, A ^{[1
]}

Eggink, G ^{[1
]}

机构：

[1] DLO, Agrotechnol Res Inst, Dept Ind Microbiol, NL-6700 AA Wageningen, Netherlands

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1998年 / 50卷 / 05期

关键词：

D O I：

10.1007/s002530051331

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A newly isolated solvent-tolerant Pseudomonas putida strain converts (+)-limonene to high concentrations of a single and stable product, perillic acid. The presence of a cosubstrate is necessary for growth and perillic acid production. Glycerol appears to be the most suitable cosubstrate among those tested. An optimal combination of 150 mM limonene and 50 mM glycerol was found. Other factors that improve the extent and/or rate of bioconversion are the use of ammonia or urea as the nitrogen source, control of temperature at 30-34 degrees C and of pH at 7.0, as well as the use of emulsifiers to increase the bioavailability of limonene. Up to 18 mM (3.0 g . l(-1)) perillic acid is produced, a concentration that is not growth inhibitory. The observations that a single product is formed in high concentrations and that it is not further metabolized make this limonene bioconversion of commercial interest.

引用

页码：538 / 544

页数：7

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