Local expression of mlgf-1 modulates ubiquitin, caspase and CDK5 expression in skeletal muscle of an ALS mouse model

Objective: The functional connection between muscle and nerve is often altered in several neuromuscular diseases, including amyotrophic lateral sclerosis (ALS). Knowledge about the molecular and cellular mechanisms involved in the restorative reactions is important to our understanding of the processes involved in neuromuscular maintenance. We previously reported that muscle-restricted expression of a localized lgf-1 isoform maintained muscle integrity, stabilized neuromuscular junctions, reduced inflammation in the spinal cord and enhanced motor neuronal survival in SODG93A mice, delaying the onset and progression of the disease. In this study, we analysed potential molecular pathways that are modulated by mlgf-1 to counteract muscle wasting and to preserve motor neurons activity. Methods: We performed molecular and morphologic analysis to address the specific proposed questions. Results and discussion: Ubiquitin expression and caspase activity resulted markedly increased in SODG93A muscle but maintained at very low levels in the SODG93A x MLC/mlgf-1 (SODG93A/mlgf-1) transgenic muscle. In addition, CDK5 expression, a serine-threonine protein kinase that has been implicated in a number of physiologic processes in nerve and muscle cells, was reduced in SODG93A muscle but increased in SODG93A x MLC/mlgf-1 muscle. Notably, while the toxic p25 protein accumulated in SODG93A muscle, no accumulation was evident in the SODG93A/ mlgf-1 muscle. The maintenance of muscle phenotype was also associated with maintenance of a normal peripheral nerve, and a greater number of myelinated axons. Conclusion: These observations offer novel insights into the role of mlgf-1 in the attenuation of muscle wasting in the mouse model of ALS disease.