Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family

被引：37

作者：

Brown, RC ^{[1
]}

Tsalavouta, M

Terzoglou, V

Magoulas, A

McAndrew, BJ

机构：

[1] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland

[2] Hellen Ctr Marine Res, Iraklion, Crete, Greece

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 03期

关键词：

cross-species amplification; gilthead seabream; microsatellites; Sparidae; Sparus aurata;

D O I：

10.1111/j.1471-8286.2005.01007.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Six new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross-species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species.

引用

页码：605 / 607

页数：3

共 7 条

[1]

Batargias C, 1999, MOL ECOL, V8, P897

[2] The molecular phylogeny of the Sparidae (Pisces, Perciformes) based on two satellite DNA families [J].

De la Herrán, R ;

Rejón, CR ;

Rejón, MR ;

Garridos-Ramos, MA .

HEREDITY, 2001, 87 (6) :691-697

[3] FSTAT (Version 1.2): A computer program to calculate F-statistics [J].

Goudet, J .

JOURNAL OF HEREDITY, 1995, 86 (06) :485-486

[4] Twelve new microsatellite markers for gilted seabream (Sparus aurata L.):: characterization, polymorphism and linkage [J].

Launey, S ;

Krieg, F ;

Haffray, P ;

Bruant, JS ;

Vanniers, A ;

Guyomard, R .

MOLECULAR ECOLOGY NOTES, 2003, 3 (03) :457-459

[5] Novel polymorphic microsatellite markers for the common pandora (Pagellus erythrinus) [J].

Ramsak, A ;

Garoia, F ;

Guarniero, I ;

Mannini, P ;

Tinti, F .

MOLECULAR ECOLOGY NOTES, 2003, 3 (04) :553-555

[6] GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].

RAYMOND, M ;

ROUSSET, F .

JOURNAL OF HEREDITY, 1995, 86 (03) :248-249

[7] Ten microsatellite loci isolated and developed for the blackspot seabream, Pagellus bogaraveo (Brunnich 1768) [J].

Stockley, BM ;

Rogers, AD ;

Iyengar, A ;

Menezes, G ;

Santos, R ;

Long, A .

MOLECULAR ECOLOGY, 2000, 9 (07) :999-1000

← 1 →