Periodate-sensitive immunological cross-reactivity between keyhole limpet haemocyanin (KLH) and serodiagnostic Schistosoma mansoni egg antigens

被引：30

作者：

Hamilton, JV

Chiodini, PL

Fallon, PG

Doenhoff, MJ ^{[1
]}

机构：

[1] Univ Wales, Sch Biol Sci, Bangor LL57 2UW, Gwynedd, Wales

[2] Hosp Trop Dis, Dept Clin Parasitol, London NW1 0PE, England

来源：

PARASITOLOGY | 1999年 / 118卷

关键词：

serodiagnosis; keyhole limpet haemocyanin (KLH); CEF6; cross-reactivity;

D O I：

10.1017/S0031182098003461

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Both CEF6, a cation-exchange fraction of soluble Schistosoma mansoni egg antigens (SEX), composed of the 2 antigens, alpha-1 and omega-1, and haemocyanin from the keyhole limper, Megathura crenulata, have shown potential for immunodiagnosis of human schistosomiasis. Possible cross-reactivity between antigens in SEA and keyhole limpet haemocyanin (KLH) was explored by Western immunoblotting and enzyme-linked immunosorbent assay (ELISA) using sera from rabbits immunized with KLH, SEA, CEF6, alpha-1, omega-1, or egg antigen k5. Both immunoassays revealed a high degree of serological cross-reactivity between the schistosome egg antigens and KLH, much of it due to sodium periodate-sensitive epitopes. Cross-reactivity with schistosome antigens with proven diagnostic efficacy may thus, in part, explain the usefulness of KLH for the diagnosis of human schistosomiasis mansoni.

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页码：83 / 89

页数：7

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