Simultaneous determination of ephedrine, pseudoephedrine, norephedrine and methylephedrine in Kampo medicines by high-performance liquid chromatography

被引：69

作者：

Okamura, N ^{[1
]}

Miki, H ^{[1
]}

Harada, T ^{[1
]}

Yamashita, S ^{[1
]}

Masaoka, Y ^{[1
]}

Nakamoto, Y ^{[1
]}

Tsuguma, M ^{[1
]}

Yoshitomi, H ^{[1
]}

Yagi, A ^{[1
]}

机构：

[1] Fukuyama Univ, Fac Pharm & Pharmaceut Sci, Hiroshima 7290292, Japan

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1999年 / 20卷 / 1-2期

关键词：

Kampo medicine; ephedrine; pseudoephedrine; norephedrine; methylephedrine;

D O I：

10.1016/S0731-7085(99)00065-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simultaneous high-performance liquid chromatographic method for the determination of ephedrine, pseudoephedrine, norephedrine and methylephedrine (ephedrine alkaloids) in Kampo medicines which contain Ephedrae Herba was established. The analysis can be accomplished within 25 min with a Wakosil-II 5C18 HG column by isocratic elution using a mixture of water, acetonitrile and sodium dodecyl sulfate (65:35:0.4) as the mobile phase at a flow-rate of 1.0 mi min(-1), and detection at 210 nm. The detection limits of ephedrine alkaloids are 0.37-1.06 mu M per injection (5 mu l). This method was applied to analyze the quantities in eight Kampo decoctions; Mao-to, Makyo-yokukan-to, Makyo-kanseki-to, Yokuinin-to, Sho-seiryu-to, Keima-kakuhan-to, Kakkon-to and Kakkon-to-ka-senkyu-sin'i. The concentration (per Ephedrae Herba gram) of ephedrine alkaloids was higher in the Makyo-kanseki-to decoction than in the others. Calcium sulfate from Gypsum Fibrosum raised ephedrine alkaloids dissolution in the Makyo-kanseki-to decoction. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：363 / 372

页数：10

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