Human embryonic stem cells

被引:10
作者
Akutsu, Hidenori [1 ]
Cowan, Chad A. [1 ]
Melton, Douglas [1 ]
机构
[1] Harvard Univ, Howard Hughes Med Inst, Cambridge, MA USA
来源
EMBRYONIC STEM CELLS | 2006年 / 418卷
关键词
D O I
10.1016/S0076-6879(06)18005-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Human embryonic stem cells hold great promise in furthering our treatment of disease and increasing our understanding of early development. This chapter describes protocols for the derivation and maintenance of human embryonic stem cells. In addition, it summarizes briefly several alternative methods for the culture of human embryonic stem cells. Thus, this chapter provides a good starting point for researchers interested in harnessing the potential of human embryonic stem cells.
引用
收藏
页码:78 / 92
页数:15
相关论文
共 44 条
[1]   Unique gene expression signatures of independently-derived human embryonic stem cell lines [J].
Abeyta, MJ ;
Clark, AT ;
Rodriguez, RT ;
Bodnar, MS ;
Pera, RAR ;
Firpo, MT .
HUMAN MOLECULAR GENETICS, 2004, 13 (06) :601-608
[2]   Human feeder layers for human embryonic stem cells [J].
Amit, M ;
Margulets, V ;
Segev, H ;
Shariki, K ;
Laevsky, I ;
Coleman, R ;
Itskovitz-Eldor, J .
BIOLOGY OF REPRODUCTION, 2003, 68 (06) :2150-2156
[3]   Feeder layer- and serum-free culture of human embryonic stem cells [J].
Amit, M ;
Shariki, C ;
Margulets, V ;
Itskovitz-Eldor, J .
BIOLOGY OF REPRODUCTION, 2004, 70 (03) :837-845
[4]   Establishment and in vitro differentiation of a new embryonic stem cell line from human blastocyst [J].
Baharvand, H ;
Ashtiani, SK ;
Valojerdi, MR ;
Shahverdi, A ;
Taee, A ;
Sabour, D .
DIFFERENTIATION, 2004, 72 (05) :224-229
[5]   Gene expression in human embryonic stem cell lines: unique molecular signature [J].
Bhattacharya, B ;
Miura, T ;
Brandenberger, R ;
Mejido, J ;
Luo, YQ ;
Yang, AX ;
Joshi, BH ;
Ginis, I ;
Thies, RS ;
Amit, M ;
Lyons, I ;
Condie, BG ;
Itskovitz-Eldor, J ;
Rao, MS ;
Puri, RK .
BLOOD, 2004, 103 (08) :2956-2964
[6]   The derivation of two additional human embryonic stem cell lines from day 3 embryos with low morphological scores [J].
Chen, H ;
Qian, K ;
Hu, J ;
Liu, DH ;
Lu, WG ;
Yang, Y ;
Wang, D ;
Yan, HJ ;
Zhang, SM ;
Zhu, GJ .
HUMAN REPRODUCTION, 2005, 20 (08) :2201-2206
[7]   Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture [J].
Cheng, LZ ;
Hammond, H ;
Ye, ZH ;
Zhan, XC ;
Dravid, G .
STEM CELLS, 2003, 21 (02) :131-142
[8]  
Cowan CA, 2004, NEW ENGL J MED, V350, P1353, DOI 10.1056/NEJMsr040330
[9]   Human embryonic stem cells as a model for early human development [J].
Dvash, T ;
Benvenisty, N .
BEST PRACTICE & RESEARCH CLINICAL OBSTETRICS & GYNAECOLOGY, 2004, 18 (06) :929-940
[10]   ESTABLISHMENT IN CULTURE OF PLURIPOTENTIAL CELLS FROM MOUSE EMBRYOS [J].
EVANS, MJ ;
KAUFMAN, MH .
NATURE, 1981, 292 (5819) :154-156