Calibration of micropipets using the bioluminescent protein aequorin

被引：13

作者：

Grosvenor, AL

Crofcheck, CL

Anderson, KW

Scott, DL

Daunert, S

机构：

[1] UNIV KENTUCKY,DEPT CHEM & MAT ENGN,LEXINGTON,KY 40506

[2] UNIV KENTUCKY,DEPT CHEM,LEXINGTON,KY 40506

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 15期

关键词：

D O I：

10.1021/ac970123a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pt. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.

引用

页码：3115 / 3118

页数：4

共 13 条

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Ashley CC, 1979, DETECTION MEASUREMEN

[2] Bioluminescence hybridization assays using recombinant aequorin. Application to the detection of prostate-specific antigen mRNA [J].