Hepatocyte polarity and the peroxisomal compartment: a comparative study

被引:22
作者
Depreter, M
Walker, T
De Smet, K
Beken, S
Kerckaert, I
Rogiers, V
Roels, F
机构
[1] Univ Ghent, Dept Human Anat Embryol Histol & Med Phys, B-9000 Ghent, Belgium
[2] GlaxoSmithKline, Cellular & Biochem Toxicol, In Vitro Models, Ware SG12 0DP, Herts, England
[3] Free Univ Brussels, Dept Toxicol, B-1090 Brussels, Belgium
来源
HISTOCHEMICAL JOURNAL | 2002年 / 34卷 / 3-4期
关键词
D O I
10.1023/A:1020990414190
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In search of factors that regulate the phenotype of the peroxisomal compartment in wild-type live parenchymal cells, we compared hepatocyte polarity to peroxisome differentiation, using adult live as the standard. Differentiation parameters were evaluated in a three-dimensional culture model (spheroid), in sandwich and monolayer primary hepatocyte cultures, and in 15.5 and 18.5-day-old foetal rat live. Peroxisomes, studied by immunohistochemistry, enzyme histochemistry, and catalase specific activity, were better differentiated depending on foetal age (day 18.5 > day 15.5) and culture type (spheroid > sandwich > monolayer). The hepatocyte polarity markers ATP-, ADP-, and AMP-hydrolysing activities were, in all models, mislocalized at the lateral plasma membrane, whereas in contrast the multidrug resistance-associated protein 2 (mrp2) antigen was always correctly immunolocalized at the apical membrane domain. In cultures, the correct secretion of fluorescein (mrp2- mediated) into bile canaliculi was observed. Bile canaliculi (branching, ultrastructure and immunolocalization of the tight-junction associated protein ZO-1), were better differentiated in 18.5 than in 15.5-day-old foetal live and in spheroid > sandwich > monolayer cultures. Our results show a parallelism between changes of the peroxisomal compartment and bile canalicular structure together with mrp2-mediated secretory function. Distinct polarization characteristics do not necessarily change simultaneously, suggesting different regulatory mechanisms.
引用
收藏
页码:139 / 151
页数:13
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