Afforestation Alters the Composition of Functional Genes in Soil and Biogeochemical Processes in South American Grasslands

被引:62
作者
Berthrong, Sean T. [1 ]
Schadt, Christopher W. [2 ]
Pineiro, Gervasio [3 ,4 ]
Jackson, Robert B. [4 ]
机构
[1] Duke Univ, Dept Biol, Univ Program Ecol, Durham, NC 27708 USA
[2] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA
[3] Univ Buenos Aires, Fac Agron, Lab Anal Reg & Teledetecc, IFEVA,CONICET, Buenos Aires, DF, Argentina
[4] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA
基金
美国国家科学基金会;
关键词
LITTER DECOMPOSITION; BIOGEOGRAPHY; MICROARRAY; EXPRESSION; DIVERSITY; BIODIVERSITY; BACTERIA; ARCHAEA;
D O I
10.1128/AEM.01126-09
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Soil microbes are highly diverse and control most soil biogeochemical reactions. We examined how microbial functional genes and biogeochemical pools responded to the altered chemical inputs accompanying land use change. We examined paired native grasslands and adjacent Eucalyptus plantations (previously grassland) in Uruguay, a region that lacked forests before European settlement. Along with measurements of soil carbon, nitrogen, and bacterial diversity, we analyzed functional genes using the GeoChip 2.0 microarray, which simultaneously quantified several thousand genes involved in soil carbon and nitrogen cycling. Plantations and grassland differed significantly in functional gene profiles, bacterial diversity, and biogeochemical pool sizes. Most grassland profiles were similar, but plantation profiles generally differed from those of grasslands due to differences in functional gene abundance across diverse taxa. Eucalypts decreased ammonification and N fixation functional genes by 11% and 7.9% (P < 0.01), which correlated with decreased microbial biomass N and more NH4+ in plantation soils. Chitinase abundance decreased 7.8% in plantations compared to levels in grassland (P = 0.017), and C polymer-degrading genes decreased by 1.5% overall (P < 0.05), which likely contributed to 54% (P < 0.05) more C in undecomposed extractable soil pools and 27% less microbial C (P < 0.01) in plantation soils. In general, afforestation altered the abundance of many microbial functional genes, corresponding with changes in soil biogeochemistry, in part through altered abundance of overall functional gene types rather than simply through changes in specific taxa. Such changes in microbial functional genes correspond with altered C and N storage and have implications for long-term productivity in these soils.
引用
收藏
页码:6240 / 6248
页数:9
相关论文
共 51 条
[1]  
[Anonymous], BIOL EUCALYPTS
[2]  
[Anonymous], 2006, FAO Forestry Paper 147
[3]   Recent advances in BNF with non-legume plants [J].
Baldani, JI ;
Caruso, L ;
Baldani, VLD ;
Goi, SR ;
Dobereiner, J .
SOIL BIOLOGY & BIOCHEMISTRY, 1997, 29 (5-6) :911-922
[4]   Amino acid cycling in three cold-temperate forests of the northeastern USA [J].
Berthrong, Sean T. ;
Finzi, Adrien C. .
SOIL BIOLOGY & BIOCHEMISTRY, 2006, 38 (05) :861-869
[5]  
BERTHRONG ST, ECOL APPL IN PRESS
[6]   Terminal restriction fragment length polymorphism data analysis for quantitative comparison of microbial communities [J].
Blackwood, CB ;
Marsh, T ;
Kim, SH ;
Paul, EA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (02) :926-932
[7]  
Brady N.C., 2002, The nature and property of soils, V13
[8]   Sediment denitrifier community composition and nirS gene expression investigated with functional gene microarrays [J].
Bulow, Silvia E. ;
Francis, Christopher A. ;
Jackson, George A. ;
Ward, Bess B. .
ENVIRONMENTAL MICROBIOLOGY, 2008, 10 (11) :3057-3069
[9]   mRNA extraction and reverse transcription-PCR protocol for detection of nifH gene expression by Azotobacter vinelandii in soil [J].
Bürgmann, H ;
Widmer, F ;
Sigler, WV ;
Zeyer, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (04) :1928-1935
[10]   Differential Responses of Nitrate Reducer Community Size, Structure, and Activity to Tillage Systems [J].
Cheneby, D. ;
Brauman, A. ;
Rabary, B. ;
Philippot, L. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (10) :3180-3186