Scanty integration of osteochondral allografts cryopreserved at low temperatures with dimethyl sulfoxide

被引:15
作者
Forriol, Francisco [2 ]
Longo, Umile Giuseppe [3 ]
Alvarez, Eduardo [4 ]
Campi, Stefano [3 ]
Ripalda, Purificacion [4 ]
Rabitti, Carla [5 ]
Maffulli, Nicola [1 ]
Denaro, Vincenzo [3 ]
机构
[1] Queen Mary Univ London, Mile End Hosp, Ctr Sports & Exercise Med, Barts & London Sch Med & Dent, London E1 4DG, England
[2] FREMAP Hosp, Res Unit, Madrid, Spain
[3] Campus Biomed Univ, Dept Orthopaed & Trauma Surg, I-00128 Rome, Italy
[4] Dept Orthopaed Surg, Lab Orthopaed Res, Pamplona, Spain
[5] Campus Biomed Univ, Dept Anat Pathol, I-00128 Rome, Italy
关键词
Osteochondral allograft; Cartilage; OARSI; ICRS; Sports; Sheep; AUTOLOGOUS CHONDROCYTE IMPLANTATION; ARTICULAR-CARTILAGE; STORED ALLOGRAFTS; KNEE-JOINT; DEFECTS; REPAIR; TRANSPLANTATION; VIABILITY; GENE; OSTEOARTHRITIS;
D O I
10.1007/s00167-010-1317-8
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
100224 [整形外科学];
摘要
To compare the integration of osteochondral allografts cryopreserved at different temperatures and different concentrations of dimethyl sulfoxide in an in vivo sheep animal model. Thirty-six adult sheep were randomly allocated to 6 groups of allograft osteochondral transplantation. Six osteochondral cylinders were stored for 6 weeks at -80A degrees C; 6 at -80A degrees C with 10% dimethyl sulfoxide (DMSO); 6 at -80A degrees C with 10% DMSO for 90 min; 6 at -186A degrees C; 6 at -186A degrees C with 10% DMSO; 6 at -186A degrees C for 90 min. After transplantation, all animals were euthanized at 6 months. Harvested specimens underwent gross morphologic and histologic evaluation. We found no statistically significant differences when comparing the gross cartilage morphology and histopathologic scores of each group. The Mankin and OARSI scores and the modified Wakitani and OARSI scores showed a good correlation grade. The Mankin and modified Wakitani scores showed a fair correlation grade. The cryopreservation protocols adopted in the present study provided scanty integration in an in vivo sheep model of osteochondral allograft transplantation. Therefore, their use in the clinical practice is discouraged.
引用
收藏
页码:1184 / 1191
页数:8
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