Analysis of Ipl1-Mediated Phosphorylation of the Ndc80 Kinetochore Protein in Saccharomyces cerevisiae

被引：52

作者：

Akiyoshi, Bungo ^{[1
,2
]}

Nelson, Christian R. ^{[1
]}

Ranish, Jeffrey A. ^{[3
]}

Biggins, Sue ^{[1
]}

机构：

[1] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA

[2] Univ Washington, Mol & Cellular Biol Program, Seattle, WA 98195 USA

[3] Inst Syst Biol, Seattle, WA 98103 USA

来源：

GENETICS | 2009年 / 183卷 / 04期

基金：

美国国家卫生研究院;

关键词：

SISTER-CHROMATID COHESION; BUDDING YEAST; MICROTUBULE ATTACHMENT; SPINDLE CHECKPOINT; KINASE; COMPLEX; BINDING; HEC1;

D O I：

10.1534/genetics.109.109041

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Phosphorylation of the Ndc80 kinetochore protein by the Ipl1/Aurora B kinase reduces its microtubule binding activity in vitro. We found that kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo, but this phosphorylation is not essential. Instead, we show that additional Ipl1 targets contribute to segregation and the spindle checkpoint.

引用

页码：1591 / 1595

页数：5

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