Osteopontin is expressed and functional in human eosinophils

被引：73

作者：

Puxeddu, I. ^{[1
,2
]}

Berkman, N. ^{[3
]}

Ribatti, D. ^{[4
]}

Bader, R.

Haitchi, H. M. ^{[2
]}

Davies, D. E. ^{[2
]}

Howarth, P. H. ^{[2
]}

Levi-Schaffer, F. ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Dept Pharmacol & Expt Therapeut, Sch Pharm, Fac Med, IL-91120 Jerusalem, Israel

[2] Southampton Gen Hosp, Div Infect Inflammat & Repair, Sch Med, Southampton SO9 4XY, Hants, England

[3] Hadassah Hebrew Univ, Med Ctr, Inst Pulmonol, Jerusalem, Israel

[4] Univ Bari, Fac Med, Dept Human Anat & Histol, Bari, Italy

来源：

ALLERGY | 2010年 / 65卷 / 02期

关键词：

airway remodeling; allergic inflammation; angiogenesis; eosinophils; osteopontin; ENDOTHELIAL GROWTH-FACTOR; INTEGRIN; ANGIOGENESIS; INFLAMMATION; DEFICIENT; MIGRATION; CELLS; LINK;

D O I：

10.1111/j.1398-9995.2009.02148.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100108 [医学免疫学];

摘要：

P>Background: Eosinophils are critically involved in allergic inflammation and tissue remodeling. Osteopontin (OPN) is a glycoprotein molecule which exhibits pro-fibrogenic and pro-angiogenic properties and has recently also been implicated in allergic diseases. In this study, we investigated the expression and function of OPN in human eosinophils. Methods: Osteopontin mRNA (RT-PCR) and protein (immunofluorescence) expression in peripheral blood eosinophils from atopic human subjects were evaluated. Soluble OPN release was determined in resting and activated eosinophils. The contribution of OPN to eosinophil-induced angiogenesis was determined using the chick embryo chorio- allantoic membrane (CAM) assay and OPN-induced eosinophil chemotaxis was determined (ChemoTx System microplate wells). Finally, OPN expression in bronchoalveolar lavage (BAL) fluids from mild asthmatic and normal control subjects was determined. Results: Osteopontin is expressed in human eosinophils and is increased following GM-CSF and IL-5 activation. Eosinophil-derived OPN contributes to eosinophil-induced angiogenesis. Recombinant OPN promotes eosinophil chemotaxis in vitro and this effect is mediated by alpha(4)beta(1) integrin binding. Soluble OPN is increased in the bronchoalveolar lavage fluid from mild asthmatic subjects and correlates with eosinophil counts. Conclusions: We therefore conclude that OPN is likely to contribute to the process of angiogenesis observed in the airways in asthma.

引用

页码：168 / 174

页数：7

共 35 条

[1]

Increased levels of vascular endothelial growth factor in induced sputum in asthmatic patients [J].

Asai, K ;

Kanazawa, H ;

Kamoi, H ;

Shiraishi, S ;

Hirata, K ;

Yoshikawa, J .

CLINICAL AND EXPERIMENTAL ALLERGY, 2003, 33 (05) :595-599

[2]

Eta-1 (osteopontin): An early component of type-1 (cell-mediated) immunity [J].

Ashkar, S ;

Weber, GF ;

Panoutsakopoulou, V ;

Sanchirico, ME ;

Jansson, M ;

Zawaideh, S ;

Rittling, SR ;

Denhardt, DT ;

Glimcher, MJ ;

Cantor, H .

SCIENCE, 2000, 287 (5454) :860-864

[3]

Altered bleomycin-induced lung fibrosis in osteopontin-deficient mice [J].

Berman, JS ;

Serlin, D ;

Li, XF ;

Whitley, G ;

Hayes, J ;

Rishikof, DC ;

Ricupero, DA ;

Liaw, L ;

Goetschkes, M ;

O'Regan, AW .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2004, 286 (06) :L1311-L1318

[4]

EXPRESSION AND DISTRIBUTION OF OSTEOPONTIN IN HUMAN TISSUES - WIDESPREAD ASSOCIATION WITH LUMINAL EPITHELIAL SURFACES [J].

BROWN, LF ;

BERSE, B ;

VANDEWATER, L ;

PAPADOPOULOSSERGIOU, A ;

PERRUZZI, CA ;

MANSEAU, EJ ;

DVORAK, HF ;

SENGER, DR .

MOLECULAR BIOLOGY OF THE CELL, 1992, 3 (10) :1169-1180

[5]

The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease [J].

Chabas, D ;

Baranzini, SE ;

Mitchell, D ;

Bernard, CCA ;

Rittling, SR ;

Denhardt, DT ;

Sobel, RA ;

Lock, C ;

Karpuj, M ;

Pedotti, R ;

Heller, R ;

Oksenberg, JR ;

Steinman, L .

SCIENCE, 2001, 294 (5547) :1731-1735

[6]

Osteopontin promotes vascular endothelial growth factor-dependent breast tumor growth and angiogenesis via autocrine and paracrine mechanisms [J].

Chakraborty, Goutam ;

Jain, Shalini ;

Kundu, Gopal C. .

CANCER RESEARCH, 2008, 68 (01) :152-161

[7]

Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival [J].

Denhardt, DT ;

Noda, M ;

O'Regan, AW ;

Pavlin, D ;

Berman, JS .

JOURNAL OF CLINICAL INVESTIGATION, 2001, 107 (09) :1055-1061

[8]

Expression and function of the vascular endothelial growth factor receptor FLT-1 in human eosinophils [J].

Feistritzer, C ;

Kaneider, NC ;

Sturn, DH ;

Mosheimer, BA ;

Kädhler, CM ;

Wiedermann, CJ .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2004, 30 (05) :729-735

[9]

Anti-IL-5 treatment reduces deposition of ECM proteins in the bronchial subepithelial basement membrane of mild atopic asthmatics [J].

Flood-Page, P ;

Menzies-Gow, A ;

Phipps, S ;

Ying, S ;

Wangoo, A ;

Ludwig, MS ;

Barnes, N ;

Robinson, D ;

Kay, AB .

JOURNAL OF CLINICAL INVESTIGATION, 2003, 112 (07) :1029-1036

[10]

Regulation of vascular calcification - Roles of phosphate and osteopontin [J].

Giachelli, CM ;

Speer, MY ;

Li, XW ;

Rajachar, RM ;

Yang, H .

CIRCULATION RESEARCH, 2005, 96 (07) :717-722

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