Antagonistic activities of epiphytic bacteria from soybean leaves against Pseudomonas syringae pv glycinea in vitro and in planta

被引:34
作者
May, R [1 ]
Volksch, B [1 ]
Kampmann, G [1 ]
机构
[1] UNIV JENA,PHARMACEUT BIOL FAC,INST MICROBIOL,D-07743 JENA,GERMANY
关键词
NUCLEATION ACTIVE BACTERIA; PHASEOLOTOXIN PRODUCTION; BIOLOGICAL-CONTROL; LEAF SURFACES; PHASEOLICOLA; METABOLITES; SPOTS;
D O I
10.1007/s002489900041
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Over two growing seasons, 273 bacterial strains were isolated from soybean leaves without and with bacterial blight symptoms caused by Pseudomonas syringae pv. glycinea (Psg). The majority of the isolates from leaves with symptoms were identified as Psg (43%), followed by Erwinia herbicola (21%), and Enterobacter/Erwinia (19%). The isolates from leaves without symptoms included mainly a group of unidentified Gram-negative bacteria (22%), Psg (21%), and E. herbicola (18%). Psg colonized the soybean leaves prior to saprophytic bacteria, and remained dominant during both seasons on healthy, as well as infected, leaves. Eighty-two saprophytic isolates were tested in vitro for their antagonistic activities against Psg, using an agar-diffusion assay. For the in planta assay, Psg and each isolate were simultaneously inoculated into wounds of pin-pricked leaves of greenhouse-grown soybean plants. Twenty-nine isolates were antagonistic in vitro. Nineteen isolates were able to suppress the growth of Psg and prevented the formation of leaf spots in planta when mixtures of isolate and pathogen were inoculated at ratios > 1. Only 9 of the 82 isolates inhibited Psg in vitro as well as in planta. Most antagonists detected belonged to the genera Pseudomonas and the species Erwinia herbicola. The in planta assay should be a reliable predictor of field performance for screening of biological control agents.
引用
收藏
页码:118 / 124
页数:7
相关论文
共 30 条
[1]  
[Anonymous], BIOL CONTROL PHYLLOP
[2]  
Beer S.V., 1984, Acta Horticulture, V151, P195, DOI DOI 10.17660/ACTAHORTIC.1984.151.24
[3]  
Blakeman J.P., 1991, J. Appl. Bacteriol, V70, P49
[4]   POTENTIAL FOR BIOLOGICAL-CONTROL OF PLANT-DISEASES ON THE PHYLLOPLANE [J].
BLAKEMAN, JP ;
FOKKEMA, NJ .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1982, 20 :167-192
[5]   NUMBERS AND ANTAGONISTIC PROPERTIES OF BACTERIA FROM BUDS OF FIELD-GROWN SOYBEAN PLANTS [J].
CHAKRAVARTI, BP ;
DAFT, GC ;
LEBEN, C .
CANADIAN JOURNAL OF MICROBIOLOGY, 1972, 18 (05) :696-+
[6]   METABOLITES OF PSEUDOMONAS INVOLVED IN THE BIOCONTROL OF PLANT-DISEASE [J].
DOWLING, DN ;
OGARA, F .
TRENDS IN BIOTECHNOLOGY, 1994, 12 (04) :133-141
[7]  
EL-GOORANI M A, 1991, Phytopathology, V81, P121
[8]  
Fahy P.C., 1983, PLANT BACTERIAL DIS, P337
[9]  
GIBBINS LN, 1978, P INT C PLANT PATHOG, V2, P403
[10]   RAPID METHOD FOR DISTINCTION OF GRAM-NEGATIVE FROM GRAM-POSITIVE BACTERIA [J].
GREGERSEN, T .
EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1978, 5 (02) :123-127