Isolation and characterization of microsatellite DNA markers in the malaria vector Anopheles funestus

被引：18

作者：

Cohuet, A

Simard, F

Berthomieu, A

Raymond, M

Fontenille, D

Weill, M

机构：

[1] Inst Rech Dev, Lab Lutte Contre Insectes Nuisibles, F-34394 Montpellier 05, France

[2] Org Coordinat Luttre Contre Endemies Afrique Cent, Yaounde, Cameroon

[3] Univ Montpellier 2, UMR CNRS 5554, CC065, Inst Sci Evolut,Lab Genet & Environm, Montpellier, France

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 04期

关键词：

Anopheles funestus; malaria; microsatellites; polymorphism;

D O I：

10.1046/j.1471-8286.2002.00290.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Screening of the Anopheles funestus genomic DNA library detected 18 new sequences with dinucleotide tandem repeats. Primers were designed to amplify the loci and 14 out of 18 gave a repeatable and scorable amplification. Deviations from Hardy Weinberg expectations were tested for each locus in a sample of 30 wild Anopheles funestus females. No heterozygote deficiency was detected for 11 loci of 14, thus revealing the absence of null alleles. The number of alleles per locus ranged from 5 to 15, and observed heterozygosity from 0.13 to 0.85.

引用

页码：498 / 500

页数：3

共 6 条

[1] A RIBOSOMAL-RNA GENE PROBE DIFFERENTIATES MEMBER SPECIES OF THE ANOPHELES-GAMBIAE COMPLEX [J].