Nutritional value of Ganoderma extract and assessment of its genotoxicity and anti-genotoxicity using comet assays of mouse lymphocytes

The nutritive composition of a hot aqueous extract of wild Ganoderma fruit bodies was determined. This extract was assessed for cytotoxicity and in vivo genotoxicity by both acute and subchronic exposure of mice (given by mouth at a dose equivalent to extract of 220 g fresh Ganoderma fruit body/kg body weight). To test any alleged protection against mutagens by Ganoderma treatments, the mice were injected intraperitoneally with the radiomimetic mutagen ethyl methanesulfonate (EMS), and after 24 hr of treatment their lymphocytes were examined using the comet assay. Ganoderma extract consisted of Folin-positive, material (68.9% of dry weight), but protein comprised only 7.3% of dry weight. Glucose accounted fur 11.1% and metals 10.2% of dry weight (K, Mg and Ca being the major components with Cc (often touted as being of value in sales literature for Ganoderma preparations) having the fifth highest metal concentration at 489 mu g/g). In comparison to rodent chow, Ganoderma extract was a modest dietary supplement, No evidence was found for genotoxic chromosomal breakage nor cytotoxic effects by Ganoderma extract in the mouse, nor did it protect against the effects of ethyl methanesulfonate. We found no support in this study for the extract having any value in protecting against the test mutagen. (C) 2000 Elsevier Science Ltd. All rights reserved.