Electrochemistry of monoazahemin reconstituted myoglobin at an indium oxide electrode

被引：23

作者：

Taniguchi, I ^{[1
]}

Mie, Y ^{[1
]}

Nishiyama, K ^{[1
]}

Brabec, V ^{[1
]}

Novakova, O ^{[1
]}

Neya, S ^{[1
]}

Funasaki, N ^{[1
]}

机构：

[1] KYOTO PHARMACEUT UNIV,DEPT PHYS CHEM,KYOTO 607,JAPAN

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1997年 / 420卷 / 1-2期

基金：

日本学术振兴会;

关键词：

myoglobin; monoazahemin; reconstituted myoglobin; indium oxide; redox potential; cyclic voltammetry;

D O I：

10.1016/S0022-0728(96)01022-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Monoazahemin reconstituted myoglobin was prepared and its electrochemical behavior was studied in comparison with native myoglobin. For both myoglobins well-defined voltammograms were clearly obtained at highly hydrophilic surfaces of indium oxide electrodes, Although monoazahemin showed a more positive redox potential than hemin (measured in methanol), monoazahemin reconstituted myoglobin showed a more negative redox potential than native myoglobin in a 50 mM bis-Tris buffer solution (pH 6.5), suggesting that for both native and reconstituted myoglobins the heme environment including proximal histidine as an axial ligand of the redox center plays an important role in determining the redox potential. Also, a unique electrochemical response of cyano-monoazahemin reconstituted myoglobin was demonstrated.

引用

页码：5 / 9

页数：5

共 20 条

[1]

[Anonymous], [No title captured]

[2] DIRECT ELECTROCHEMISTRY OF REDOX PROTEINS [J].