Towards practical soft X-ray spectromicroscopy of biomaterials

被引：57

作者：

Hitchcock, AP ^{[1
]}

Morin, C ^{[1
]}

Heng, YM ^{[1
]}

Cornelius, RM ^{[1
]}

Brash, JL ^{[1
]}

机构：

[1] McMaster Univ, Brockhouse Inst Mat Res, Hamilton, ON L8S 4M1, Canada

来源：

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 2002年 / 13卷 / 08期

关键词：

D O I：

10.1163/156856202320401960

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Scanning transmission X-ray microscopy (STXM) is being developed as a new tool to study the surface chemical morphology and biointeractions of candidate biomaterials with emphasis on blood compatible polymers. STXM is a synchrotron based technique which provides quantitative chemical mapping at a spatial resolution of 50 nm. Chemical speciation is provided by the near edge X-ray absorption spectral (NEXAFS) signal. We show that STXM can detect proteins on soft X-ray transparent polymer thin films with monolayer sensitivity. Of great significance is the fact that measurements can be made in situ, i.e. in the presence of an overlayer of the protein solution. The strengths, limitations and future potential of STXM for studies of biomaterials are discussed.

引用

页码：919 / 937

页数：19

共 28 条

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