Study of the factors affecting the extraction of soybean protein by reverse micelles

被引：40

作者：

Zhao, Xihong ^{[1
]}

Li, Yanmei ^{[2
]}

He, Xiaowei ^{[1
]}

Zhong, Nanjing ^{[1
]}

Xu, Zhenbo ^{[1
,3
]}

Yang, Liansheng ^{[1
]}

机构：

[1] S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China

[2] Guangzhou Med Univ, Guangzhou 510182, Guangdong, Peoples R China

[3] Univ Maryland, Dept Microbial Pathogenesis, Sch Dent, Baltimore, MD 21201 USA

来源：

MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 02期

关键词：

Reverse micelles; Extraction; Soybean protein; Sodium di-(2-ethlhexyl) sulfosuccinate (AOT); LIQUID-LIQUID-EXTRACTION; BACK-EXTRACTION; SOLUBILIZATION; PURIFICATION; PEROXIDASE; RECOVERY;

D O I：

10.1007/s11033-009-9515-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In this work, the forward and back extraction of soybean protein by reverse micelles was studied. The reverse micellar systems were formed by anionic surfactant sodium bis(2-ethyl hexyl) sulfosuccinate (AOT), isooctane and KCl solution. The effects of AOT concentration, aqueous pH, KCl concentration and phase volume ratio on the extraction efficiency of soybean protein were tested. Suitability of reverse micelles of AOT and Triton-X-100/AOT mixture in organic solvent toluene for soybean protein extraction was also investigated. The experimental results lead to complete forward extraction at the AOT concentration 120 mmol l(-1), aqueous pH 5.5 and KCl concentration 0.8 mol l(-1). The backward extraction with aqueous phase (pH 5.5) resulted in 100% extraction of soybean protein from the organic phase.

引用

页码：669 / 675

页数：7

共 27 条

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[3]

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[4]

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