Identification of organic anion transporting polypeptide 4 (Oatp4) as a major full-length isoform of the liver-specific transporter-1 (rlst-1) in rat liver

被引：124

作者：

Cattori, V

Hagenbuch, B

Hagenbuch, N

Stieger, B

Ha, R

Winterhalter, KE

Meier, PJ ^{[1
]}

机构：

[1] Univ Zurich Hosp, Dept Med, Div Clin Pharmacol & Toxicol, CH-8091 Zurich, Switzerland

[2] ETH Zurich, Swiss Fed Inst Technol, Dept Biol, Inst Biochem, CH-8092 Zurich, Switzerland

[3] Univ Zurich Hosp, Dept Med, Cardiovasc Therapy Res Grp, CH-8091 Zurich, Switzerland

来源：

FEBS LETTERS | 2000年 / 474卷 / 2-3期

关键词：

liver transport; bile salt; sulfobromophthalein; steroid conjugate; thyroid hormone; leukotriene C4;

D O I：

10.1016/S0014-5793(00)01596-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel organic anion transporting polypeptide (Oatp)4(1) was isolated from rat liver that is 35 amino acids longer than the reported rat liver specific organic anion transporter (rlst)-1 and exhibits a 64% amino acid sequence identity with the human OATP-C (LST-1/OATP2; gene symbol SLC21A6). When expressed in Xenopus laevis oocytes, Oatp4 (Slc21a10) mediated polyspecific uptake of a variety of organic anions including taurocholate (K(m)similar to 27 mu M), bromosulfophthalein (K(m)similar to 1.1 mu M) and steroid conjugates, Based on nuclease protection analysis Oatp4 appears to be the predominant transcript in rat liver indicating that rlst-1 plays a minor role in overall hepatic organic anion uptake. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：242 / 245

页数：4

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