Detection of Coxiella burnetii in market chicken eggs and mayonnaise

被引：16

作者：

Tatsumi, Noriyuki

Baumgartner, Andreas

Qiao, Ying

Yamamoto, Ikkyu

Yamaguchi, Kazuo

机构：

[1] Inst Prevent Med Zoonosis, Oyabe, Toyama 9320133, Japan

[2] Kanazawa Natl Univ, Div Funct Gen Adv, Ctr Sci Res, Kanazawa, Ishikawa, Japan

来源：

CENTURY OF RICKETTSIOLOGY: EMERGING, REEMERGING RICKETTSIOSES, MOLECULAR DIAGNOSTICS, AND EMERGING VETERINARY RICKETTSIOSES | 2006年 / 1078卷

关键词：

Coxiella burnetii; nested PCR; real-time PCR; egg; mayonnaise;

D O I：

10.1196/annals.1374.096

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We tried to detect C. burnetii in market chicken eggs and mayonnaise by nested PCR assay. The PCR target was the com 1 gene of C. burnetii. The positive rate for egg and mayonnaise samples was 4.2% and 17.6%, respectively. Direct sequence of some of the positive egg samples shows mutations whereas no mutation was found in the positive mayonnaise samples. The number of molecules of the Q fever agent is estimated at 10(4) to 10(6) per egg, according to our quantitative PCR test.

引用

页码：502 / 505

页数：4

共 4 条

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[2]

TO H, 1995, MICROBIOL IMMUNOL, V39, P663

[3] Clinical evaluation of a new PCR assay for detection of Coxiella burnetii in human serum samples [J].