Entamoeba histolytica:: sequence conservation of the Gal/GalNAc lectin from clinical isolates

被引：23

作者：

Beck, DL

Tanyuksel, M

Mackey, AJ

Haque, R

Trapaidze, N

Pearson, WR

Loftus, B

Petri, WA ^{[1
]}

机构：

[1] Univ Virginia, Dept Internal Med, Charlottesville, VA 22908 USA

[2] Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 USA

[3] Gulhane Mil Med Acad, Dept Microbiol & Clin Microbiol, TR-06018 Ankara, Turkey

[4] Int Ctr Diarrhoeal Dis Res, Dhaka 1000, Bangladesh

[5] Natl Ctr Dis Control Georgia, GE-38077 Tbilisi, Georgia

[6] Univ Virginia, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA

[7] Inst Genom Res, Rockville, MD 20850 USA

[8] Univ Virginia, Dept Pathol, Charlottesville, VA 22908 USA

来源：

EXPERIMENTAL PARASITOLOGY | 2002年 / 101卷 / 2-3期

关键词：

D O I：

10.1016/S0014-4894(02)00113-3

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The Gal/GalNAc lectin gene of Entamoeba histolytica is a major amebic virulence protein responsible for interaction with host tissues. We investigated sequence differences in the Gal/GalNAc lectin heavy subunit in three isolates from Bangladesh and one isolate from Georgia, each of which was determined to be genetically distinct by SREHP AluI digestion. Interestingly, we observed only slight genetic diversity in the lectin gene as compared with the HM1:IMSS laboratory strain, originally a clinical isolate from Mexico. Genetic conservation of the Gal/GalNAc lectin between isolates may reflect that the lectin is under strong functional selection or possibly, that E histolytica is a clonal population. Sequence conservation of the lectin indicates that immune responses against it should be cross-protective.

引用

页码：157 / 163

页数：7

共 25 条

[1] Accuracy and power of the likelihood ratio test in detecting adaptive molecular evolution [J].