Succession of cellular states in a Salmonella typhimurium population during starvation in artificial seawater microcosms

被引：73

作者：

Joux, F ^{[1
]}

Lebaron, P ^{[1
]}

Troussellier, M ^{[1
]}

机构：

[1] UNIV MONTPELLIER 2,LAB HYDROBIOL MARINE & CONTINENTALE,UMR CNRS 5556,F-34095 MONTPELLIER 05,FRANCE

来源：

FEMS MICROBIOLOGY ECOLOGY | 1997年 / 22卷 / 01期

关键词：

Salmonella typhimurium; survival; seawater; cellular states; fluorescent probes; flow cytometry;

D O I：

10.1111/j.1574-6941.1997.tb00357.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The succession of the physiological states of Salmonella typhimurium cells under starvation-survival conditions in artificial seawater was investigated by now cytometry and epifluorescence microscopy using direct examination of different cell functions. Measurements of substrate responsiveness (DVC method), real and potential respiration (redox dye 5-cyano-2,3-ditolyl tetrazolium chloride, CTC), membrane permeability (BacLight(TM) kit), as well as DNA content (Hoechst 33342 staining) reveal an important heterogeneity within the population, and suggest a progressive physiological cell alteration throughout the starvation process. The transition steps between culturable and prelytic cells are described.

引用

页码：65 / 76

页数：12

共 32 条

[1] EFFECT OF VISIBLE-LIGHT ON PROGRESSIVE DORMANCY OF ESCHERICHIA-COLI-CELLS DURING THE SURVIVAL PROCESS IN NATURAL FRESH-WATER [J].