Morphologic study of neuronal death, glial activation, and progenitor cell division in the hippocampus of rat models of epilepsy

被引：36

作者：

Tooyama, I ^{[1
]}

Bellier, JP ^{[1
]}

Park, M ^{[1
]}

Minnasch, P ^{[1
]}

Uemura, S ^{[1
]}

Hisano, T ^{[1
]}

Iwami, M ^{[1
]}

Aimi, Y ^{[1
]}

Yasuhara, O ^{[1
]}

Kimura, H ^{[1
]}

机构：

[1] Shiga Univ Med Sci, Mol Neurosci Res Ctr, Otsu, Shiga 5202192, Japan

来源：

EPILEPSIA | 2002年 / 43卷

关键词：

kindling; kainic acid; progenitor cell; microglia; DNA fragmentation;

D O I：

10.1046/j.1528-1157.43.s.9.10.x

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Purpose: To clarify the relationship of neuronal death to cellular responses, we studied neuronal death as well as reactions of glia and progenitor cells in the hippocampus of two rat models of epilepsy. Methods: Seizures were induced by either kainic acid (KA) administration or electrical kindling. Neuronal degeneration was assessed by in situ DNA fragmentation analysis. Reactions of glial cells were studied by immunohistochemistry. Progenitor cell division was evaluated using the bromodeoxyuridine (BrdU) labeling method. Results: DNA fragmentation and reactive microglia were observed in the CA1, CA3, and hilus region for 24 h to 4 weeks after KA injection, but not detected in the kindling model. Reactive astrocytes and enhancement of progenitor cell division were seen in both animal models. The number of BrdU-positive cells began to increase on day 3 after KA injection, peaked on day 5, and returned to baseline on day 10. After kindling, the number of BrdU-positive cells began to increase after five consecutive experience of stage I seizures. Conclusions: These observations show that neuronal degeneration is not necessary for triggering the upregulation. Microglial activation is closely related to the neuronal death process induced by KA.

引用

页码：39 / 43

页数：5

共 19 条

[1] REACTIVE MICROGLIA IN HIPPOCAMPAL SCLEROSIS ASSOCIATED WITH HUMAN TEMPORAL-LOBE EPILEPSY [J].