Gene discovery in the freshwater fish parasite Trypanosoma carassii:: Identification of trans-sialidase-like and mucin-like genes

被引：17

作者：

Agüero, F

Campo, V

Cremona, L

Jäger, A

Di Noia, JM

Overath, P

Sánchez, DO

Frasch, AC ^{[1
]}

机构：

[1] Univ Nacl Gen San Martin, CONICET, Inst Tecnol Chascomus, Inst Invest Biotecnol, RA-1650 Buenos Aires, DF, Argentina

[2] Max Planck Inst Biol, Abt Membranbiochem, D-72076 Tubingen, Germany

来源：

INFECTION AND IMMUNITY | 2002年 / 70卷 / 12期

关键词：

D O I：

10.1128/IAI.70.12.7140-7144.2002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A total of 1,921 expressed sequence tags (ESTs) were obtained from bloodstream trypomastigotes of Trypanosoma carassii, a parasite of economic importance due to its high prevalence in fish farms. Analysis of the data set allowed us to identify a trans-sialidase (TS)-like gene and three ESTs coding for putative mucin-like genes. TS activity was detected in cell extracts of bloodstream trypomastigotes. We have also used the sequence information obtained to identify genes that have not been previously described in trypanosomatids. (Additional information on these ESTs can be found at http://genoma.unsam.edu.ar/projects/tca.).

引用

页码：7140 / 7144

页数：5

共 23 条

[1] A random sequencing approach for the analysis of the Trypanosoma cruzi genome:: General structure, large gene and repetitive DNA families, and gene discovery [J].

Agüero, F ;

Verdún, RE ;

Frasch, ACC ;

Sánchez, DO .

GENOME RESEARCH, 2000, 10 (12) :1996-2005

[2] Highly purified glycosylphosphatidylinositols from Trypanosoma cruzi are potent proinflammatory agents [J].

Almeida, IC ;

Camargo, MM ;

Procópio, DO ;

Silva, LS ;

Mehlert, A ;

Travassos, LR ;

Gazzinelli, RT ;

Ferguson, MAJ .

EMBO JOURNAL, 2000, 19 (07) :1476-1485

[3] Tandem amino acid repeats from Trypanosoma cruzi shed antigens increase the half-life of proteins in blood [J].

Buscaglia, CA ;

Alfonso, J ;

Campetella, O ;

Frasch, ACC .

BLOOD, 1999, 93 (06) :2025-2032

[4] Structural basis of sialyltransferase activity in trypanosomal sialidases [J].

Buschiazzo, A ;

Tavares, GA ;

Campetella, O ;

Spinelli, S ;

Cremona, ML ;

París, G ;

Amaya, MF ;

Frasch, ACC ;

Alzari, PM .

EMBO JOURNAL, 2000, 19 (01) :16-24

[5] Life without transcriptional control? From fly to man and back again [J].

Clayton, CE .

EMBO JOURNAL, 2002, 21 (08) :1881-1888

[6] A SINGLE TYROSINE DIFFERENTIATES ACTIVE AND INACTIVE TRYPANOSOMA-CRUZI TRANS-SIALIDASES [J].

CREMONA, ML ;

SANCHEZ, DO ;

FRASCH, ACC ;

CAMPETELLA, O .

GENE, 1995, 160 (01) :123-128

[7] The Trypanosoma cruzi mucin family is transcribed from hundreds of genes having hypervariable regions [J].

Di Noia, JM ;

D'Orso, I ;

Åslund, L ;

Sánchez, DO ;

Frasch, ACC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (18) :10843-10850

[8] THE PROTOZOAN TRYPANOSOMA-CRUZI HAS A FAMILY OF GENES RESEMBLING THE MUCIN GENES OF MAMMALIAN-CELLS [J].

DINOIA, JM ;

SANCHEZ, DO ;

FRASCH, ACC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (41) :24146-24149

[9] Functional diversity in the trans-sialidase and mucin families in Trypanosoma cruzi [J].

Frasch, ACC .

PARASITOLOGY TODAY, 2000, 16 (07) :282-286

[10]

GERBER LD, 1992, J BIOL CHEM, V267, P12168

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