Genetic characterization and diversity of Rathayibacter toxicus

被引:15
作者
Agarkova, I. V.
Vidaver, A. K.
Postnikova, E. N.
Riley, I. T.
Schaad, N. W. [1 ]
机构
[1] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD USA
[2] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA
[3] Univ Adelaide, Adelaide, SA, Australia
关键词
D O I
10.1094/PHYTO-96-1270
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Rathayibacter toxicus is a nematode-vectored gram-positive bacterium responsible for a gumming disease of grasses and production of a highly potent animal and human toxin that is often fatal to livestock and has a history of occurring in unexpected circumstances. DNA of 22 strains of R. toxicus from Australia were characterized using amplified fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE). AFLP analysis grouped the 22 strains into three genetic clusters that correspond to their geographic origin. The mean similarity between the three clusters was 85 to 86%. PFGE analysis generated three different banding patterns that enabled typing the strains into three genotypic groups corresponding to the same AFLP clusters. The similarity coefficient was 63 to 81% for XbaI and 79 to 84% for SpeI. AFLP and PFGE analyses exhibited an analogous level of discriminatory power and produced congruent results. PFGE analysis indicated that the R. toxicus genome was represented by a single linear chromosome, estimated to be 2.214 to 2.301 Mb. No plasmids were detected.
引用
收藏
页码:1270 / 1277
页数:8
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