Dynamics of cyclic GMP synthesis in retinal rods

被引：185

作者：

Burns, ME ^{[1
]}

Mendez, A

Chen, J

Baylor, DA

机构：

[1] Stanford Univ, Med Ctr, Dept Neurobiol, Stanford, CA 94305 USA

[2] Univ So Calif, Keck Sch Med, Doheny Eye Inst, Mary D Allen Lab Vis Res, Los Angeles, CA 90033 USA

[3] Univ So Calif, Keck Sch Med, Dept Ophthalmol, Los Angeles, CA 90033 USA

[4] Univ So Calif, Keck Sch Med, Dept Cell & Neurobiol, Los Angeles, CA 90033 USA

[5] Univ Calif Davis, Dept Psychiat, Davis, CA 95616 USA

[6] Univ Calif Davis, Ctr Neurosci, Davis, CA 95616 USA

来源：

NEURON | 2002年 / 36卷 / 01期

关键词：

D O I：

10.1016/S0896-6273(02)00911-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In retinal rods, Ca2+ exerts negative feedback control on cGMP synthesis by guanylate cyclase (GC). This feedback loop was disrupted in mouse rods lacking guanylate cyclase activating proteins GCAP1 and GCAP2 (GCAPs(-/-)). Comparison of the behavior of wild-type and GCAPs(-/-) rods allowed us to investigate the role of the feedback loop in normal rod function. We have found that regulation of GC is apparently the only Ca2+ feedback loop operating during the single photon response. Analysis of the rods' light responses and cellular dark noise suggests that GC normally responds to light-driven changes in [Ca2+] rapidly and highly cooperatively. Rapid feedback to GC speeds the rod's temporal responsiveness and improves its signal-to-noise ratio by minimizing fluctuations in cGMP.

引用

页码：81 / 91

页数：11

共 56 条

[1] THE PHOTOCURRENT, NOISE AND SPECTRAL SENSITIVITY OF RODS OF THE MONKEY MACACA-FASCICULARIS [J].

BAYLOR, DA ;

NUNN, BJ ;

SCHNAPF, JL .

JOURNAL OF PHYSIOLOGY-LONDON, 1984, 357 (DEC) :575-607

[2] DETECTION AND RESOLUTION OF VISUAL-STIMULI BY TURTLE PHOTORECEPTORS [J].

BAYLOR, DA ;

HODGKIN, AL .

JOURNAL OF PHYSIOLOGY-LONDON, 1973, 234 (01) :163-198

[3] 2 COMPONENTS OF ELECTRICAL DARK NOISE IN TOAD RETINAL ROD OUTER SEGMENTS [J].

BAYLOR, DA ;

MATTHEWS, G ;

YAU, KW .

JOURNAL OF PHYSIOLOGY-LONDON, 1980, 309 (DEC) :591-621

[4] Onset of feedback reactions underlying vertebrate rod photoreceptor light adaptation [J].

Calvert, PD ;

Ho, TW ;

LeFebvre, YM ;

Arshavsky, VY .

JOURNAL OF GENERAL PHYSIOLOGY, 1998, 111 (01) :39-51

[5] Two temporal phases of light adaptation in retinal rods [J].

Calvert, PD ;

Govardovskii, VI ;

Arshavsky, VY ;

Makino, CL .

JOURNAL OF GENERAL PHYSIOLOGY, 2002, 119 (02) :129-145

[6] EXTRUSION OF CALCIUM FROM ROD OUTER SEGMENTS IS DRIVEN BY BOTH SODIUM AND POTASSIUM GRADIENTS [J].

CERVETTO, L ;

LAGNADO, L ;

PERRY, RJ ;

ROBINSON, DW ;

MCNAUGHTON, PA .

NATURE, 1989, 337 (6209) :740-743

[7] Slowed recovery of rod photoresponse in mice lacking the GTPase accelerating protein RGS9-1 [J].

Chen, CK ;

Burns, ME ;

He, W ;

Wensel, TG ;

Baylor, DA ;

Simon, MI .

NATURE, 2000, 403 (6769) :557-560

[8] BLEACHED PIGMENT ACTIVATES TRANSDUCTION IN ISOLATED RODS OF THE SALAMANDER RETINA [J].

CORNWALL, MC ;

FAIN, GL .

JOURNAL OF PHYSIOLOGY-LONDON, 1994, 480 :261-279

[9]

Detwiler PB, 2000, METHOD ENZYMOL, V316, P133

[10] Constitutive activation of photoreceptor guanylate cyclase by Y99C mutant of GCAP-1 - Possible role in causing human autosomal dominant cone degeneration [J].

Dizhoor, AM ;

Boikov, SG ;

Olshevskaya, EV .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (28) :17311-17314

← 1 2 3 4 5 6 →