A site-specific recombinase (RinQ) is required to exert incompatibility towards the symbiotic plasmid of Rhizobium etli

被引:10
作者
Quintero, V [1 ]
Cevallos, MA [1 ]
Dávila, G [1 ]
机构
[1] Univ Nacl Autonoma Mexico, Ctr Invest Fijac Nitrogeno, Programa Evoluc Mol, Cuernavaca 62210, Morelos, Mexico
关键词
D O I
10.1046/j.1365-2958.2002.03205.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The replication/partition region of the symbiotic plasmid p42d of Rhizobium etli CE3 is characterized by the presence of the repABC operon. A recombinant plasmid containing this region is able to replicate in a R. etli derivative cured from p42d, with the same stability and copy number shown by the parental plasmid. However, when this construct is introduced into the wild-type strain, instead of exerting incompatibility against the p42d, it forms a stable cointegrate with it. In this paper, we show that a site-specific resolvase, and its action sites are essential factors to displace the symbiotic p42d. We propose a model for this novel incompatibility mechanism.
引用
收藏
页码:1023 / 1032
页数:10
相关论文
共 47 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   A NOVEL ROLE FOR SITE-SPECIFIC RECOMBINATION IN MAINTENANCE OF BACTERIAL REPLICONS [J].
AUSTIN, S ;
ZIESE, M ;
STERNBERG, N .
CELL, 1981, 25 (03) :729-736
[3]   Identification of the partitioning site within the repABC-type replicon of the composite Paracoccus versutus plasmid pTAV1 [J].
Bartosik, D ;
Szymanik, M ;
Wysocka, E .
JOURNAL OF BACTERIOLOGY, 2001, 183 (21) :6234-6243
[4]   Molecular and functional analysis of pTAV320, a repABC-type replicon of the Paracoccus versutus composite plasmid pTAV1 [J].
Bartosik, D ;
Baj, J ;
Wlodarczyk, M .
MICROBIOLOGY-UK, 1998, 144 :3149-3157
[5]  
BEYNON JL, 1980, J GEN MICROBIOL, V120, P421
[6]  
BREWIN NJ, 1980, J GEN MICROBIOL, V120, P413
[7]   ANALYSIS OF THE MULTIMER RESOLUTION SYSTEM ENCODED BY THE PARCBA OPERON OF BROAD-HOST-RANGE PLASMID RP4 [J].
EBERL, L ;
KRISTENSEN, CS ;
GIVSKOV, M ;
GROHMANN, E ;
GERLITZ, M ;
SCHWAB, H .
MOLECULAR MICROBIOLOGY, 1994, 12 (01) :131-141
[8]   Molecular basis of symbiosis between Rhizobium and legumes [J].
Freiberg, C ;
Fellay, R ;
Bairoch, A ;
Broughton, WJ ;
Rosenthal, A ;
Perret, X .
NATURE, 1997, 387 (6631) :394-401
[9]   The composite genome of the legume symbiont Sinorhizobium meliloti [J].
Galibert, F ;
Finan, TM ;
Long, SR ;
Pühler, A ;
Abola, P ;
Ampe, F ;
Barloy-Hubler, F ;
Barnett, MJ ;
Becker, A ;
Boistard, P ;
Bothe, G ;
Boutry, M ;
Bowser, L ;
Buhrmester, J ;
Cadieu, E ;
Capela, D ;
Chain, P ;
Cowie, A ;
Davis, RW ;
Dréano, S ;
Federspiel, NA ;
Fisher, RF ;
Gloux, S ;
Godrie, T ;
Goffeau, A ;
Golding, B ;
Gouzy, J ;
Gurjal, M ;
Hernandez-Lucas, I ;
Hong, A ;
Huizar, L ;
Hyman, RW ;
Jones, T ;
Kahn, D ;
Kahn, ML ;
Kalman, S ;
Keating, DH ;
Kiss, E ;
Komp, C ;
Lalaure, V ;
Masuy, D ;
Palm, C ;
Peck, MC ;
Pohl, TM ;
Portetelle, D ;
Purnelle, B ;
Ramsperger, U ;
Surzycki, R ;
Thébault, P ;
Vandenbol, M .
SCIENCE, 2001, 293 (5530) :668-672
[10]   MOLECULAR CHARACTERIZATION OF THE RESOLVASE GENE, RES, CARRIED BY A MULTICOPY PLASMID FROM CLOSTRIDIUM-PERFRINGENS - COMMON EVOLUTIONARY ORIGIN FOR PROKARYOTIC SITE-SPECIFIC RECOMBINASES [J].
GARNIER, T ;
SAURIN, W ;
COLE, ST .
MOLECULAR MICROBIOLOGY, 1987, 1 (03) :371-376