Apoptosis inducing and differentiation enhancement effect of oridonin on the all-trans-retinoic acid-sensitive and -resistant acute promyelocytic leukemia cells

被引：25

作者：

Gao, F. ^{[2
]}

Tang, Q. ^{[3
]}

Yang, P. ^{[2
]}

Fang, Y. ^{[2
]}

Li, W. ^{[4
]}

Wu, Y. ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Key Lab Cell Differentiat & Apoptosis, Dept Pathophysiol,Natl Minist Educ, Shanghai 200025, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp Affiliated 3, Shanghai 200025, Peoples R China

[3] Shanghai Acad Agr Sci SAAS, Inst Edible Fungi, Shanghai, Peoples R China

[4] Wenzhou Med Coll, Sch Life Sci, Zhejiang Prov Key Lab Med Genet, Wenzhou, Zhejiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY | 2010年 / 32卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Oridonin; apoptosis; ROS; leukemia; differentiation; OXYGEN SPECIES ROS; ANTITUMOR-ACTIVITY; BINDING-PROTEINS; RAR-ALPHA; DITERPENOIDS; INHIBITION; MATURATION; MECHANISM;

D O I：

10.1111/j.1751-553X.2009.01147.x

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

We investigated the effects of oridonin (Ori), a diterpenoid isolated from Rabdosia rubescens, on apoptosis and differentiation of all-trans-retinoic acid (ATRA)-sensitive (NB4) and ATRA-resistant (NB4-R1) cells. The results showed that reactive oxygen species initiates Ori-induced apoptosis. In addition, we found that neither Ori nor ATRA (10 nm) alone induced marked cell differentiation, while co-treatment of these two compounds can induce differentiation of NB4 and NB4-R1 cells which was accompanied by increased RAR alpha, C/EBP epsilon or C/EBP beta. This is the first report to show that RAR alpha could be accumulated by Ori which may be useful as a probe to investigate the mechanism of RAR alpha catabolism. These results suggest that Ori is a potential candidate for acute promyelocytic leukemia cancer therapy.

引用

页码：E114 / E122

页数：9

共 30 条

[1]

Chen S, 2005, INT J ONCOL, V26, P579

[2]

P53-mediated cell cycle arrest and apoptosis through a caspase-3-independent, but caspase-9-dependent pathway in oridonin-treated MCF-7 human breast cancer cells [J].