Immobilization of organic solvent-soluble lipase in nonaqueous conditions and properties of the immobilized enzymes

被引：18

作者：

Fukunaga, K

Minamijima, N

Sugimura, Y

Zhang, ZZ

Nakao, K

机构：

[1] Dept. of Appl. Chem. and Chem. Eng., Fac. of Eng., Yamaguchi Univ., T.

来源：

JOURNAL OF BIOTECHNOLOGY | 1996年 / 52卷 / 02期

关键词：

Pseudomonas lipase; immobilization; photo-crosslinkable prepolymer; lipid-coated enzyme; enzymatic esterifications;

D O I：

10.1016/S0168-1656(96)01630-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipid-coated Pseudomonas lipase was successfully immobilized by entrapping with hydrophobic, photo-crosslinkable, resin prepolymers under non-aqueous conditions. We studied the feasibility of using this new type of biocatalyst, which is an immobilized organic solvent-soluble enzyme, for enzymatic synthesis in organic solvent. Esterification of lauric acid with benzyl alcohol in benzene and transesterification between racemic sulcatol and isopropenyl acetate in different organic solvents were carried out with the immobilized lipid-coated lipase as the model reactions. In both reaction systems, the immobilized organic solvent-soluble enzyme was observed to have excellent activity and stability. (C) 1996 Elsevier Science B.V. All rights reserved.

引用

页码：81 / 88

页数：8

共 17 条

[1] USE OF BIOLOGICAL-SYSTEMS FOR THE PREPARATION OF CHIRAL MOLECULES .3. AN APPLICATION IN PHEROMONE SYNTHESIS - PREPARATION OF SULCATOL ENANTIOMERS [J].