A new method for studying gradient-induced neutrophil desensitization based on an open microfluidic chamber

被引：56

作者：

Keenan, Thomas M. ^{[1
]}

Frevert, Charles W. ^{[2
,3
]}

Wu, Aileen ^{[4
]}

Wong, Venus ^{[2
,3
]}

Folch, Albert ^{[4
]}

机构：

[1] Stem Cell & Regenerat Med Ctr, Madison, WI USA

[2] Univ Washington, Dept Med, Seattle, WA 98195 USA

[3] Vet Affairs Med Ctr, Med Res Serv, Seattle, WA 98195 USA

[4] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA

来源：

LAB ON A CHIP | 2010年 / 10卷 / 01期

关键词：

IN-VITRO; INTERLEUKIN-8; MIGRATION; CHEMOTAXIS; ADHESION; MECHANISMS; GENERATOR;

D O I：

10.1039/b913494h

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

During inflammation neutrophils rapidly migrate to the site of tissue damage or infection by following complex gradients of bacterial peptides and host-derived chemokines. The efficiency and speed of neutrophil migration is critically dependent upon the ability of neutrophils to sense new gradients and utilize only those that provide the most direct path to the damaged or infected site. Receptor desensitization plays an important role in migration efficiency and is most commonly studied using bath application of chemotactic factor solutions instead of presenting cells with gradients analogous to those they would experience in vivo. Here we describe a new method for examining gradient-induced neutrophil desensitization using a previously-developed open-chamber microfluidic gradient generator.

引用

页码：116 / 122

页数：7

共 33 条

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