Molecular analysis of a mobilizable theta-mode trimethoprim resistance plasmid from coagulase-negative staphylococci

被引：33

作者：

Apisiridej, S ^{[1
]}

Leelaporn, A ^{[1
]}

Scaramuzzi, CD ^{[1
]}

Skurray, RA ^{[1
]}

Firth, N ^{[1
]}

机构：

[1] UNIV SYDNEY,SCH BIOL SCI,SYDNEY,NSW 2006,AUSTRALIA

来源：

PLASMID | 1997年 / 38卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1006/plas.1997.1292

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Staphylococcus epidermidis plasmid pSK639 is the prototype of a newly described family of small plasmids identified in clinical staphylococcal isolates. pSK639 is 8 kb in length and possesses a composite structure consisting of an IS257-flanked segment mediating trimethoprim resistance (Tp(r)), and regions responsible for replication and mobilization of the plasmid. Comparative sequence analysis suggests that a pSK639-like plasmid may represent a progenitor of previously identified staphylococcal Tp(r) determinants related to the transposon-like structure, Tn4003. In contrast to the small staphylococcal plasmids characterized to date that all utilize a rolling circle mode of replication, the replication region of pSK639 was found to contain features typical of an iteron-controlled theta-mode replicon. pSK639 is the first small plasmid of this type to be identified in the staphylococci. (C) 1997 Academic Press.

引用

页码：13 / 24

页数：12

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[1] DETERMINATION OF RESTRICTION SITES AND NUCLEOTIDE-SEQUENCE SURROUNDING RELAXATION SITE OF CO1E1 [J].