Mitochondrial import of the ADP/ATP carrier:: The essential TIM complex of the intermembrane space is required for precursor release from the TOM complex

被引:86
作者
Truscott, KN
Wiedemann, N
Rehling, P
Müller, H
Meisinger, C
Pfanner, N
Guiard, B
机构
[1] Univ Freiburg, Inst Biochem & Mol Biol, D-79104 Freiburg, Germany
[2] Univ Freiburg, Fak Biol, D-79104 Freiburg, Germany
[3] Univ Paris 06, CNRS, Ctr Genet Mol, Lab Propre, F-91190 Gif Sur Yvette, France
关键词
D O I
10.1128/MCB.22.22.7780-7789.2002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mitochondrial intermembrane space contains a protein complex essential for cell viability, the Tim9-Tim10 complex. This complex is required for the import of hydrophobic membrane proteins, such as the ADP/ATP carrier (AAC), into the inner membrane. Different views exist about the role played by the Tim9-Tim10 complex in translocation of the AAC precursor across the outer membrane. For this report we have generated a new tim10 yeast mutant that leads to a strong defect in AAC import into mitochondria. Thereby, for the first time, authentic AAC is stably arrested in the translocase complex of the outer membrane (TOM), as shown by antibody shift blue native electrophoresis. Surprisingly, AAC is still associated with the receptors Tom70 and Tom20 when the function of Tim10 is impaired. The nonessential Tim8-Tim13 complex of the intermembrane space is not involved in the transfer of AAC across the outer membrane. These results define a two-step mechanism for translocation of AAC across the outer membrane. The initial insertion of AAC into the import channel is independent of the function of Tim9-Tim10; however, completion of translocation across the outer membrane, including release from the TOM complex, requires a functional Tim9-Tim10 complex.
引用
收藏
页码:7780 / 7789
页数:10
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