The development of a bioengineered organ germ method

被引：330

作者：

Nakao, Kazuhisa

Morita, Ritsuko

Saji, Yasumitsu

Ishida, Kentaro

Tomita, Yusuke

Ogawa, Miho

Saitoh, Masahiro

Tomooka, Yasuhiro

Tsuji, Takashi ^{[1
]}

机构：

[1] Tokyo Univ Sci, Dept Biol Sci & Technol, Fac Ind Sci & Technol, Noda, Chiba 2788510, Japan

[2] Tokyo Univ Sci, Tissue Engn Res Ctr, Noda, Chiba 2788510, Japan

[3] Osaka Univ, Dept Mol & Cellular Biochem, Grad Sch Dent, Osaka, Japan

来源：

NATURE METHODS | 2007年 / 4卷 / 03期

关键词：

D O I：

10.1038/nmeth1012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To bioengineer ectodermal organs such as teeth and whisker follicles, we developed a three-dimensional organ-germ culture method. The bioengineered tooth germ generated a structurally correct tooth, after both in vitro organ culture as well as transplantation under a tooth cavity in vivo, showing penetration of blood vessels and nerve fibers. Our method provides a substantial advance in the development of bioengineered organ replacement strategies and regenerative therapies.

引用

页码：227 / 230

页数：4

共 15 条

[1] Tissue engineering, stem cells and cloning: current concepts and changing trends [J].