HPLC-tandem mass spectrometric method to characterize resveratrol metabolism in humans

被引:92
作者
Urpi-Sarda, Mireia
Zamora-Ros, Raul
Lamuela-Raventos, Rosa
Cherubini, Antonio
Jauregui, Olga
De la Torre, Rafael
Isabel Covas, Maria
Estruch, Ramon
Jaeger, Walter
Andres-Lacueva, Cristina
机构
[1] Univ Barcelona, Pharm Sch, Nutr & Food Sci Dept, Barcelona, Spain
[2] Univ Perugia, Sch Med, Dept Clin & Expt Med, Inst Gerontol & Geriatr, Perugia, Italy
[3] Univ Barcelona, Inst Invest Biomed August Pi Sunyer, Sci & Tech Serv, Barcelona, Spain
[4] Univ Barcelona, Hosp Clin, Dept Internal Med, Barcelona, Spain
[5] Inst Municipal Invest Med, Pharmcol Res Unit, Barcelona, Spain
[6] Inst Municipal Invest Med, Lipids & Cardiovasc Epidemiol Unit, Barcelona, Spain
[7] Univ Vienna, Dept Clin Pharm & Diagnost, Vienna, Austria
关键词
D O I
10.1373/clinchem.2006.071936
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Nutritional biomarkers are alternatives to traditional dietary assessment tools. We sought to develop a method for nutritional analysis of resveratrol, a phenolic compound with purported health-promoting properties, and to determine all resveratrol metabolites. Methods: We obtained LDL and urine samples from 11 healthy male volunteers who had consumed 250 mL of Merlot red wine; We measured resveratrol and its metabolites with 96-well solid-phase extraction plates coupled with HPLC-tandem mass spectrometry. Hexestrol was used as the internal standard. Gradient chromatography in multiple reaction monitoring mode was performed on a Luna C-18 column, maintained at 40 degrees C; m/z transitions were as follows: resveratrol, 227/185; resveratrol glucosides, 389/227; resveratrol glucuronides, 403/ 227; resveratrol sulfates, 307/227; taxifolin, 303/285; and hexestrol, 269/134. Results: Standard calibration curves were linear at 4.4-3289.5 nmol/L. Residual analyses were 100% (3.2) for trans-resveratrol and 100% (11.1) for trans-piceid. In both matrices, imprecision (CV) was < 10.8% at all concentrations. Detection limits for resveratrol were 0.2 nmol/L (1,130, 0.3 nmol/L (synthetic urine), and 4.0 nmol/L (blank urine). Resveratrol and metabolites were checked for stability, and no degradation was observed. Conclusions: The HPLC-tandem mass spectrometry method enabled us to identify resveratrol sulfates in human LDL and to characterize the complete profile of resveratrol metabolism in human LDL and urine. This method provides an accurate index of exposure to resveratrol and its metabolites, which can be used as nutritional biomarkers for evaluating the biological effects of moderate wine intake on human health. (c) 2007 American Association for Clinical Chemistry
引用
收藏
页码:292 / 299
页数:8
相关论文
共 33 条
[1]  
Andlauer W, 2000, DRUG EXP CLIN RES, V26, P47
[2]   Quantification of mycophenolic acid and glucuronide metabolite in human serum by HPLC-tandem mass spectrometry [J].
Annesley, TM ;
Clayton, LT .
CLINICAL CHEMISTRY, 2005, 51 (05) :872-877
[3]   Inhibition of cancer growth by resveratrol is related to its low bioavailability [J].
Asensi, M ;
Medina, I ;
Ortega, A ;
Carretero, J ;
Baño, MC ;
Obrador, E ;
Estrela, JM .
FREE RADICAL BIOLOGY AND MEDICINE, 2002, 33 (03) :387-398
[4]   Regioselective and stereospecific glucuronidation of trans- and cis-resveratrol in human [J].
Aumont, V ;
Krisa, S ;
Battaglia, E ;
Netter, P ;
Richard, T ;
Mérillon, JM ;
Magdalou, J ;
Sabolovic, N .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2001, 393 (02) :281-289
[5]   Therapeutic potential of resveratrol:: the in vivo evidence [J].
Baur, Joseph A. ;
Sinclair, David A. .
NATURE REVIEWS DRUG DISCOVERY, 2006, 5 (06) :493-506
[6]  
Corder R, 2001, NATURE, V414, P863, DOI 10.1038/414863a
[7]   Unique properties of polyphenol stilbenes in the brain:: More than direct antioxidant actions;: Gene/protein regulatory activity [J].
Doré, S .
NEUROSIGNALS, 2005, 14 (1-2) :61-70
[8]   VALIDATION OF THE MINNESOTA LEISURE-TIME PHYSICAL-ACTIVITY QUESTIONNAIRE IN SPANISH MEN [J].
ELOSUA, R ;
MARRUGAT, J ;
MOLINA, L ;
PONS, S ;
PUJOL, E ;
ARQUER, A ;
COVAS, MI ;
DEFLORES, T ;
FORNELLS, X ;
MARTINEZ, S ;
PEREA, M ;
PUJOL, P ;
RICHART, JA ;
RUBIES, J ;
TOMAS, A .
AMERICAN JOURNAL OF EPIDEMIOLOGY, 1994, 139 (12) :1197-1209
[9]   INHIBITION OF HUMAN LDL OXIDATION BY RESVERATROL [J].
FRANKEL, EN ;
WATERHOUSE, AL ;
KINSELLA, JE .
LANCET, 1993, 341 (8852) :1103-1104
[10]   Absorption of three wine-related polyphenols in three different matrices by healthy subjects [J].
Goldberg, DA ;
Yan, J ;
Soleas, GJ .
CLINICAL BIOCHEMISTRY, 2003, 36 (01) :79-87